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Agrociencia
versão On-line ISSN 2521-9766versão impressa ISSN 1405-3195
Agrociencia vol.41 no.6 Texcoco Ago./Set. 2007
Protección Vegetal
Susceptibilidad de Helicoverpa zea (Boddie) a la δ-endotoxina Cry2Ab de Bacillus thuringiensis berliner
1 Campus Montecillo. Colegio de Postgraduados. Montecillo, Estado de México. México. (popolocas@hotmail.com) (concho@colpos.mx).
2 Facultad de Agronomía. Universidad Autónoma de San Luis Potosí. Álvaro Obregón 64. 78000. San Luis Potosí, San Luis Potosí.
3 Campo Experimental Valle del Yaqui. CIR Noroeste, INIFAP. Sonora, México.
4 USDA-ARS Southern Insect Management Research Unit. 141 Experiment Station Rd., Stoneville, MS 38776.
5 Campus Veracruz. Colegio de Postgraduados. México.
El algodonero Bollgard II® expresa las δ-endotoxinas Cry1Ac y Cry2Ab de Bacillus thuringiensis Berliner, controla larvas de Helicoverpa zea (Boddie) y es probable que en un futuro cercano se empiece a utilizar en la agricultura mexicana. Sin embargo, se desconoce la susceptibilidad inicial a esta toxina en poblaciones de H. zea. El objetivo de este estudio fue determinar la susceptibilidad a la δ-endotoxina Cry2Ab en larvas neonatas de cinco poblaciones (una susceptible y cuatro de campo) de H. zea. Los niveles de mortalidad observados fueron bajos (<43%) en las dosis evaluadas (≤5 μg mL-1). La concentración estimada de toxina necesaria para impedir que 50% de las larvas expuestas llegara al tercer ínstar o inhibición de desarrollo del 50% de larvas tratadas (ID50) varió de 0.017 a 0.086 μg mL-1, mientras que la ID95 varió de 1.06 a 1.93 μg mL-1. La concentración que redujo o inhibió 50% del peso de larvas tratadas (IP50) varió de 0.013 a 0.025 μg mL-1, y la IP95 de 0.5 a 1.48 μg mL-1. Los valores que se obtuvieron de toxicidad a la δ-endotoxina Cry2Ab en larvas de H. zea se consideran como respuesta inicial o punto de referencia, dado que dicha toxina aún no se utiliza en campo y servirán para estimar la proporción de cambio a través del tiempo una vez que el algodonero Bollgard II® se utilice en México.
Palabras clave: Algodonero transgénico; Bollgard II®; Cry2Ab2; gusano elotero; línea base
Bollgard II® cotton expresses the δ-endotoxins Cry1Ac and Cry2Ab of Bacillus thuringiensis Berliner, controls larvae of Helicoverpa zea (Boddie), and will probably be used in the near future in Mexican agriculture. However, the initial susceptibility to this toxin in populations of H. zea is unknown. The objective of the present study was to determine the susceptibility to the δ-endotoxin Cry2Ab in neonate larvae of five populations (one susceptible and four field populations) of H. zea. The observed mortality levels were low (<43%) in the evaluated doses (≤5 μg mL-1). The estimated concentration necessary to prevent 50% of the exposed larvae from reaching the third instar or inhibition of development of 50% of treated larvae (ID50) varied from 0.017 to 0.086 μg mL-1, while the ID95 varied from 1.06 to 1.93 μg mL-1. The concentration that reduced or inhibited 50% of the weight of treated larvae (IP50) varied from 0.013 to 0.025 μg mL-1, and the IP95 from 0.5 to 1.48 μg mL-1. The obtained toxicity values to δ-endotoxin Cry2Ab in larvae of H. zea are considered as initial response or reference point, given that this toxin is still not used in the field and will serve to estimate the proportion of change over time once Bolgard II® is used in México.
Key words: Transgenic cotton; Bollgard II®; Cry2Ab2; corn borer; base line
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Recibido: Mayo de 2006; Aprobado: Junio de 2007
Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons
