SciELO - Scientific Electronic Library Online

vol.22 número1Aspectos biológicos, ecológicos, epidemiológicos y manejo de Candidatus LiberibacterModelo de humedad en un invernadero semicerrado índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados

  • No hay artículos similaresSimilares en SciELO


Revista Chapingo. Serie horticultura

versión On-line ISSN 2007-4034versión impresa ISSN 1027-152X

Rev. Chapingo Ser.Hortic vol.22 no.1 Chapingo ene./abr. 2016 

Scientific article

Evaluation of post-harvest applications of gibberellic acid on the quality and shelf life of three varieties of Mexican lime

Laura Olivia Zea-Hernández1 

Crescenciano Saucedo-Veloz1  * 

Nicacio Cruz-Huerta1 

Martha Elva Ramírez-Guzmán1 

Manuel Marciano Robles-González2 

1Colegio de Postgraduados. Carretera México-Texcoco km 36.5, Montecillo, Texcoco, Estado de México, C.P. 56230, MÉXICO.

2 Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Campo Experimental Tecomán. Carretera Colima-Manzanillo km 35, Tecomán, Colima, C.P. 28100, MÉXICO.


Among the acid limes produced in Mexico, the Mexican lime is the most important in the domestic market; however, because of the heterogeneous quality of the fruit at harvest time, the export volume is limited. Due to the thin epicarp, the fruits are sensitive to weight and green color loss, so their shelf life is short. Three varieties have recently been registered, namely Colimex, Colimón and Lise, whose response to the use of plant hormones to slow senescence and maintain quality is unknown. The aim of this research was to study the effect of pre-harvest treatment with gibberellic acid (GA3) on the quality and shelf life of the fruit of the above-mentioned varieties. In two periods of fruit growth, spraying with GA3 (30 mg∙L-1) was performed. After the harvest, a wax was applied to fruits with and without GA3; in addition, there were fruits without any treatment (control). The fruits were stored for 10 days at 22 ± 2 °C. At the end of this time, the following variables were evaluated: weight loss, color index, and concentration of chlorophyll, citric acid, TSS and ascorbic acid. AG3 treatments decreased weight loss and delayed changes in color, chlorophyll, TSS and citric acid, with ascorbic acid remaining unchanged. This response was more consistent in the Colimex and Lise varieties. We conclude that pre-harvest treatment with GA3, in combination with wax, maintains fruit quality for a longer period.

Keywords: Citrus aurantifolia Swingle; color index; ascorbic acid; weight loss; citric acid


Entre la producción de limas ácidas en México, la del limón mexicano es la de mayor importancia en el mercado nacional; sin embargo, por lo heterogéneo de la calidad del fruto al momento de la cosecha, el volumen de exportación es limitado. Debido a lo sutil del epicarpio, los frutos son sensibles a pérdidas de peso y del color verde, siendo corta su vida de anaquel. Recientemente se han registrado tres variedades: Colimex, Colimón y Lise, cuya respuesta al uso de fitohormonas para retardar la senescencia y mantener la calidad, es desconocida. El objetivo fue estudiar el efecto del tratamiento, precosecha con ácido giberélico (AG3), en la calidad y vida de anaquel del fruto de las variedades señaladas. En dos periodos de crecimiento del fruto, se realizaron aspersiones con AG3 (30 mg∙L-1). Tras la cosecha, a frutos con y sin AG3 se les aplicó una cera, considerando además otro sin ningún tratamiento (testigo). Los frutos se almacenaron por 10 días en 22 ± 2 °C. Al término se evaluaron las variables: pérdida de peso, índice de color, concentración de clorofila y de ácido cítrico, SST y ácido ascórbico. Los tratamientos con AG3 disminuyeron las pérdidas de peso y retardaron los cambios en color, clorofila, SST y ácido cítrico, permaneciendo sin cambios el ácido ascórbico. Esta respuesta fue más consistente en las variedades Colimex y Lise. Se concluyó que el tratamiento precosecha con AG3, en combinación con encerado, mantiene la calidad de los frutos por mayor tiempo.

Palabras clave: Citrus aurantifolia Swingle; índice de color; ácido ascórbico; pérdidas de peso; ácido cítrico.


Globally, Mexican lime (Citrus aurantifolia Swingle) is produced in several countries, including Mexico, Brazil, India, Peru and Egypt (Plattner, 2014). The juice is used in the preparation of drinks and the peel for the extraction of pectin, whereas the essential oils are used in the perfume industry (Liu, Heying, & Tanumihardjo, 2012). Nutritionally, Mexican lime is important for its contribution of ascorbic acid (vitamin C), minerals, citric acid and bioactive compounds such as limonin glucoside, a highly-oxygenated triterpenoid associated with anticancer activity (Jacob, Hasegawa, & Manners, 2000).

In Mexico, production of Mexican lime, with spines or criollo (a native, regional variety), is estimated at 1.4 million tons (Servicio de Información Agroalimentaria y Pesquera [SIAP], 2014), which is mainly sold in the domestic market, since only 2.3 % of production meets the color, size, juice content and health characteristics required for export (Plattner, 2014). The fruits of Mexican lime with spines are small, with a high number of seeds, a thin epicarp and accelerated senescence; the latter results in a short shelf life, so a high percentage of the harvested fruit is destined for either industrial purposes or the fresh market.

Based on studies of natural genetic variation, conducted by the National Institute for Forestry, Agriculture and Livestock Research (INIFAP) through its Mexican Lime Breeding Program, three registered varieties have been generated: Colimex, Lise and Colimón. The first are trees with spines, which have high yield and larger fruit size; the second is similar to the first but in trees without spines, and the third produces seedless fruits (Robles-González, Carrillo-Medrano, Manzanilla- Ramírez, Velázquez-Monreal, & Medina-Urrutia, 2010). In addition, the fruits of these varieties have better quality characteristics in terms of juice content, flavor components and refrigerated storage potential (Muñoz- Lazcano, Saucedo-Veloz, García-Osorio, & Robles- González, 2011); however, their response to the use of bioregulators with phytohormonal effect, aimed at reducing the advance of deteriorative changes related to senescence, which significantly affect the quality of the fruits and limit their shelf life, is still unknown.

Physiologically, the fruit has non-climacteric behavior (Kader, 2000), so in postharvest it shows changes associated with the phenomenon of senescence which, among other processes, involves a decrease in photosynthetic capacity and chlorophyll content, vitamin C losses and changes in membrane permeability, which favor the loss of water in cells and tissues (Goldschmidt, 2000). These processes represent the main factors that significantly affect the quality and reduce the shelf life of Mexican lime fruits (Álvarez-Armenta et al., 2010). In this regard, significant losses in the internal and external quality of untreated fruits of the Colimex, Lise and Colimon varieties, stored at 22 ± 2 °C for six days, have been reported (Muñoz- Lazcano et al., 2011).

The use of plant hormones such as auxins (Agustí et al., 2002), gibberellins (Jomori-Lye, Kluge, & Jacomino, 2003) and cytokinins (Baéz-Sañudo, Tadeo, Primo-Millo, & Zacarías, 1993) has been studied to assess their effect on the slowing of senescence-related changes in citrus fruits. In these fruits, pre-harvest applications with gibberellic acid (GA3) have been evaluated in order to delay senescence, increase epicarp firmness, delay the harvest and control physiological disorders, all with contrasting results due to the effect of species, variety, dose, and application method, among other factors (Ritenour, Burton, & McCollum, 2005). The aim of this research was to study the effect of pre-harvest treatment with GA3 on controlling the progress of senescence in the fruit of three Mexican lime varieties, and its effect on fruit quality and shelf life.

Materials and methods

This study used Mexican lime fruits of the Colimex, Lise and Colimón varieties, obtained from the Tecomán Experimental Station, Colima, Mexico, belonging to the Institute for Forestry, Agriculture and Livestock Research (INIFAP), located at 32 masl, with a BS1(h’) climate, considered as warm semi-dry, with an average temperature of 26 °C and mean annual rainfall of 750 mm.

Prior to harvest, two groups of three trees each were formed; gibberellic acid (GA3) was applied to the first group when the fruits reached 30 ± 2 mm in diameter; applications were made to the second group on two occasions, when the fruits were 20 ± 2 mm and 30 ± 2 mm in diameter. A third group of six trees remained untreated. The GA3 solution with a concentration of 30 mg∙L-1 was prepared with granulated ACTIVOL® 40 % GS; Penetrator® Plus at a ratio of 10 mL∙L-1 was used as surfactant. At 9:00 am the fruits were sprayed with 10 L of this solution per tree.

The harvest of the fruits, with and without GA3, was carried out when they reached 39 ± 2 mm in diameter (in accordance with paragraphs 4 and 5 of the Mexican Standard: NMX-FF-087-SCFI-2001, Secretaría de Agrícultura, Ganadería, Desarrollo Rural, Pesca y Alimentación [SAGARPA], 2014). After 12 h they were transferred to the laboratory where they were conditioned for 12 h at room temperature, and subsequently selected on the basis of overall health.

Based on the above, the factors studied in this research were:

Number of pre-harvest GA3 applications (zero, one or two). When two applications occurred, the fruits reached 20 ± 2 mm in diameter in the first, and 30 ± 2 mm in the second. In the case of one application, the fruits had 30 ± 2 mm in diameter.

Application of water-based wax in postharvest (with and without). This treatment consisted of immersing the fruits into the water-based wax (14 % Carnauba wax solids); then they were dried with airflow at room temperature.

From these levels, four treatments were generated (with a sample size of 60 fruits), which were: a pre-harvest GA3 application and postharvest waxing (1-GA3), two GA3 applications and waxing (2-GA3), postharvest waxing and without any treatment (control).

Subsequently, the fruits were stored for 10 days (22 ± 2 °C and 60 ± 5 % relative humidity), after which the following variables were determined:

Fruit weight loss (%). It was obtained with respect to initial fruit weight.

Color index (CI). This parameter was determined in whole fruit using the expression CI = 1000a / bL (Jiménez, Cuquerella, & Martínez, 1981); parameters L, a, b were obtained with a HunterLab Model D25A optical sensor system, Reston, Virginia, USA.

Chlorophyll concentration (mg∙100 g-1). It was obtained from the epicarp by spectrometry, performing the extraction with acetone (AOAC, 1990).

Titratable acidity (% with respect to citric acid).

Total soluble solids (TSS, in %). It was determine by the methods described by AOAC (1990).

Ascorbic acid concentration (mg ascorbic acid∙100 mL-1). It was determined by the 2,6-dichlorophenol-indophenol method (AOAC, 1980).

Determination of non-destructive variables, CI and weight loss, as well as destructive ones: chlorophyll, citric acid, TSS and ascorbic acid. It was performed on a sample composed of five replications of four fruits each. An analysis of variance based on a factorial treatment design in a completely randomized arrangement was performed, comparing the means of the treatments by the Tukey test (P ≤ 0.05) using the Statistical Analysis System statistical package (SAS, 2002).

Results and discussion

Significant differences in weight loss due to the treatment effect were observed (Table 1), being lower in fruits treated with one or two GA3 applications compared to those treated with wax and those in the control. Several researchers (Báez-Sañudo et al., 1993; Tafolla-Arellano, González-León, Tiznado-Hernández, Zacarías-García, & Báez-Sañudo, 2013) have reported the cuticle’s role in regulating water loss in fruits, as well as the effect, in Clementine mandarin (Citrus reticulata [Hort] Ex Tanaka, cv. Nules), of GA3 treatments in maintaining the permeability of the flavedo cuticles by preventing their breaking due to changes in the lipid fraction related to senescence, which results in reducing water losses by transpiration; this allows GA3 to take similar action in Mexican lime fruits.

Table 1 Effect of pre-harvest treatment with GA3 and waxing in postharvest on Mexican lime fruits stored at 22 ± 2 °C for 10 days. 

Treatment Weight loss (%) Color index TSS (%) Citric acid (%) Vitamin C (mg ascorbic acid∙100 mL-1) Chlorophyll concentration (mg∙100 g-1)
Control / Testigo 9.28a* -10.61a 7.22b 7.68a 35.41a 0.73b
Wax / Cera 8.90a -11.78a 7.18b 7.26b 34.96a 0.74b
AG3(1)+Wax / AG3(1)+Cera 6.99b -15.92b 7.56a 7.69a 39.85a 0.85a
AG3(2)+Wax / AG3(2)+Cera 6.64b -16.05b 7.53a 7.78a 39.26a 0.87a
HSD / DMSH 1.04 1.64 0.14 0.36 7.02 0.10

*Means with the same letters in each column do not differ statistically (Tukey, P≤ 0.05).

HSD: honestly significant difference.

Regarding the variety effect, Colimón fruits had the greatest weight loss (Table 2), a response suggested in the hypothesis concerning differences in changes related to the lipid fraction of cuticular waxes among the lime varieties studied. On the other hand, it has been reported that Mexican lime fruits with a weight loss of less than 6 - 7 % are suitable for marketing purposes (Muñoz-Lazcano et al., 2011), which allows establishing that only GA3-treated fruits had this condition after the established storage period and temperature. The variety × treatment interaction (Table 3) indicated that the lower weight loss corresponded to the treatments with GA3, establishing that a single application was favorable for reducing this problem.

Table 2 Effect of pre-harvest applications with GA3 and waxing in postharvest on fruits of three Mexican lime varieties stored at 22 ± 2 °C for 10 days. 

Variety Weight loss (%) Color index TSS (%) Citric acid (%) Vitamin C (mg ascorbic acid∙100 mL-1) Chlorophyll concentration (mg∙100 g-1)
Colimón 8.76a* -11.09a 7.29b 7.43a 38.88a 0.69b
Colimex 7.85b -14.80b 7.45a 7.66a 39.33a 0.85a
Lise 7.25b -14.88b 7.36a 7.71a 33.89a 0.84a
HSD / DMSH 0.82 1.29 0.1 0.28 5.5 0.09

*Means with the same letters in each column are statistically equal (Tukey, P ≤ 0.05).

HSD: honestly significant difference.

Table 3 Effect of Variety × Treatment interaction on Mexican lime fruits with and without GA3 application and stored at 22 ± 2 °C for 10 days. 

Variety × Treatment Weight loss (%) Color index TSS (%) Citric acid Vitamin C (mg ascorbic acid∙100 mL-1) Chlorophyll concentration (mg∙100 g-1)
Colimex AG3(1)+W / AG3(1)+C 5.96g* -16.35ef 7.66ab 7.86 a 40.5d 0.90a
Colimex AG3(2)+W / AG3(2)+C 6.06g -17.30f 7.63a 7.56a 42.7c 0.87a
Colimex Wax / Cera 9.10c -14.42de 7.26ab 7.36a 36.9f 0.83a
Colimex Control / Testigo 10.29a -11.14bc 7.23bc 7.83a 37.3e 0.83a
Colimón AG3(1)+W / AG3(1)+C 8.37d -14.14de 7.46ab 7.43a 43.6b 0.77ab
Colimón AG3(2)+W / AG3(2)+C 7.33e -14.22de 7.40ab 7.76a 44.4a 1.03a
Colimón Wax / Cera 9.38c -9.27ab 7.13c 7.16a 34.7h 0.5bc
Colimón Control / Testigo 9.97b -6.71a 7.23c 7.36a 32.9j 0.47c
LISE AG3(1)+W / AG3(1)+C 6.65f -17.26f 7.53ab 7.76a 34.7h 0.87ab
LISE AG3(2)+W / AG3(2)+C 6.54f -16.61ef 7.56ab 8.0a 36.0g 0.7ab
LISE Wax / Cera 8.22d -13.99bc 7.13c 7.23a 33.3i 0.90a
LISE Control / Testigo 7.57e -11.66bc 7.2c 7.83a 31.6k 0.90a
HSD / DMSH 2.94 1.89 0.41 0.89 0.05 0.32

*Means with the same letters in each column are statistically equal (Tukey, P ≤ 0.05).

HSD: honestly significant difference.

The CI results revealed significant differences due to the effect of the treatments, with the fruits treated with one or two GA3 applications being those that after 10 days of storage at 22 ± 2 °C had values corresponding to a greener tone, which was confirmed when the treatments showed a higher chlorophyll concentration in the epicarp compared to those that were only waxed and the control (Table 1). This allows assuming that the pre-harvest application of GA3, at the dose and fruit growth stages established, is effective in slowing the degradation of chlorophyll due to the effect of the progress of senescence in postharvest, thus prolonging shelf life.

Increases in chlorophyll content and delay of senescence due to GA3 applications in citrus as have been reported by several researchers studying ways to delay the harvest (García-Luís, Herrero-Villén, & Guardiola, 1992; Mcdonald, Greany, Shaw, & Mccollum, 1997; Porat et al., 2001). On the other hand, the fruits of the Colimex and Lise varieties presented, significantly, CI values corresponding to a greener tone, compared to the Colimón variety. This suggests that the latter has greater metabolic activity in postharvest, resulting in a faster degradation of chlorophyll; the lowest concentration of this pigment quantified in this variety confirms this response (Table 2). The results of the variety . treatment interaction (Table 3) confirm the positive effect of GA3 applications in slowing the loss of the epicarp’s green color, with only one application being effective.

The statistical analysis showed that, in treatments with GA3, TSS increased significantly compared to the waxed fruit and the control (Tables 1 and 3). For its part, the percentage of TSS was higher in the Colimex and Lise varieties than in Colimón (Tables 2 and 3). As in other citruses, sugar accumulation in Mexican lime fruits occurs during growth and maturation, by the source-demand mechanism (Iglesias et al., 2007). Postharvest, the sugar content tends to decrease with advancing senescence, due to the interconversion of sugars to other compounds (Yun et al, 2013.), which explains the higher TSS concentration in GA3-treated fruits due to presenting less progress of this physiological event.

In relation to citric acid, treatments with GA3 and waxing had, after 10 days at 22 ± 2 °C, a significantly higher concentration compared to the control (Table 1). It has been reported (El-Otmani & Coggins, 1991) that, in citruses, treatment with GA3 applied pre-harvest decreases the postharvest loss of citric acid; this same behavior has been observed in Mexican lime fruits with GA3 applications and stored after harvest, without waxing, at 9 ± 1 °C for 35 days (Álvarez et al., 2010). It should be noted that no significant differences among varieties were observed (Table 2); the same occurred with the variety x treatment interaction (Table 3), so it is assumed that the treatment did not affect the concentration of this compound.

As for the ascorbic acid concentration, although no significant differences between the treatment and variety factors were observed (Tables 1 and 2), the variety . treatment interaction (Table 3) showed that the fruits with GA3 applications significantly increased their concentration of this vitamin, particularly with two applications; moreover, this response was more evident in the Colimex and Lise varieties. It has been noted that, in postharvest, the decrease in ascorbic acid occurs due to, among other factors, conditions that favor water loss (Lee & Kader, 2000), a response that was evidenced by the fruits of the Colimex and Lise varieties, which had less weight loss.


Pre-harvest treatments with GA3, at a concentration of 30 mg∙L-1, in fruits of Mexican lime varieties Colimex, Colimón and Lise, with one (20 ± 2 mm in diameter) or two applications (20 ± 2 and 30 ± 2 mm in diameter), waxed postharvest (14 % Carnauba) and stored at 20 ± 2 °C for 10 days, slow the progress of senescence by presenting less physiological weight loss, an epicarp with a higher chlorophyll concentration and greener tone, and a higher concentration of citric acid and TSS in juice. For cost reasons, a single application is recommended. The treatment with two applications has a higher concentration of ascorbic acid, which increases the nutritional quality. Delay of senescence, as a function of the above-mentioned parameters, is more effective in the Colimex and Lise varieties, keeping, therefore, their quality longer, resulting in a longer shelf life compared to the Colimón variety.


Agustí, M., Zaragoza, S., Iglesias, D. J., Almela, V., Primo- Millo, E., & Talón, M. (2002). The synthetic auxin 3,5,6-TPA stimulates carbohydrate accumulation and growth in citrus fruit. Plant Growth Regulation, 36(2), 141-147. doi: 10.1023/A:1015077508675 [ Links ]

Álvarez-Armenta, R., Saucedo-Veloz, C., Chávez-Franco, S., Medina-Urrutia, V., Colinas-León, M. T., & Báez-Sañudo, R. (2010). Aplicación de ácido giberélico en precosecha y cera en postcosecha a frutos de limón mexicano. Revista Mexicana en Ciencias Agrícolas, 1(1), 95- 100. Recuperado de ]

AOAC(1980). Official Methods of Analysis of AOAC. 13th, Horwitz, W. (Ed). Washington D. C. USA. 746 p. [ Links ]

AOAC (1990). Official Methods of Analysis of AOAC. 15th, Sidney W. (Ed). Washington, D. C. USA. 1094 p. [ Links ]

Baéz-Sañudo, R., Tadeo, F. R., Primo-Millo, E., & Zacarías, L. (1993). Physiological and ultrastructural changes during the ripening and senescence of Clementine mandarin. Acta Horticulturae, 343, 18-24. doi: 10.17660/ActaHortic.1993.343.4 [ Links ]

El-Otmani, M. & Coggins, C. W. (1991). Growth regulator effects on retention of quality of stored citrus fruits. Scientia Horticultura, 45(3-4), 261-272. doi: 10.1016/0304-4238(91)90072-7 [ Links ]

García-Luís, A., Herrero-Villén, A., & Guardiola, J. L. (1992). Effects of applications of gibberellic acid on late growth, maturation and pigmentation of the Clementine mandarin. Scientia Horticulturae, 49(1-2), 71-81. doi: 10.1016/0304-4238(92)90144-2 [ Links ]

Goldschmidt, E. E. (2000). Maturation of citrus fruit: Hormonal and molecular regulation of chlorophyll breakdown and other processes. Proceedings Internacional Society of Citriculture, 1, 364-366. Recuperado de ]

Iglesias, D. J., Cercós, M., Colmenero-Flore, J. M., Naranjo, M. A., Ríos, G., Carrera, E., Ruíz-Rivero, O., Lliso, I., Morillon, R., Tadeo, F. R., & Talon, M. (2007). Physiology of citrus fruiting. Brazilian Journal of Plant Physiology, 19(4), 333-362. Recuperado de ]

Jacob, R., Hasegawa, S., & Manners, G. (2000). The potential of Citrus Limonoids as Anticancer Agents. Perishables Handling Quarterly, 102, 6-8. Recuperado de ]

Jiménez-Cuesta, M., Cuquerella, J., & Martínez-Javaga, J.M. (1982). Determination of color index for fruit degreening. Proceedings of the International Society of Citriculture, 2, 750-753. Recuperado de ]

Jomori-Lye, M. L., Kluge, A. R., & Jacomino, A. P. (2003). Cold storage of ‘Tahiti’ lime treated with 1-Metylcyclopropene. Scientia Agrícola, 60(4), 785-788. doi: 10.1590/S0103-90162003000400027 [ Links ]

Kader, A. A. (2000). Quality of horticultural products. Acta Horticulturae, 517, 17-18. Recuperado de ]

Lee, S. K., & Kader, A. A. (2000). Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biology and Technology, 20(3), 207-220. doi: 10.1016/S0925-5214(00)00133-2 [ Links ]

Liu, Y., Heying, E., & Tanumihardjo, S. A. (2012). History, global distribution and nutritional importance of citrus fruits. Comprehensive Reviews in Food Science and Food Safety, 11(6), 530-545. doi: 10.1111/j.1541-4337.2012.00201.x [ Links ]

Mcdonald, R. E., Greany, P. D., Shaw, P. E., & Mccollum, T. G. (1997). Preharvest applications of gibberellic acid delay senescence of Florida grapefruit. Journal. Horticultural Science, 72(3), 461-468. doi: 10.1080/14620316.1997.11515534 [ Links ]

Muñoz-Lazcano, A. A., Saucedo-Veloz, C., García-Osorio, C., & Robles-González, M. (2011). Evaluación de la calidad y tiempo de almacenamiento del fruto de tres variedades de limón mexicano. Revista Iberoamericana de Tecnología Postcosecha, 12(2), 156-163. Recuperado de [ Links ]

Plattner, K. (2014). Fresh market limes. Fruits and tree nuts outlook: economic insight. Economic Research Service, USDA, 9p. Recuperado de [ Links ]

Porat, R., Feng, X., Huberman, M., Galili, D., Goren, R., & Goldschmidt, E. E. (2001). Gibberellic acid slows postharvest degreening of `oroblanco` citrus fruits. HortScience, 36(5), 937-940. Recuperado de ]

Ritenour, M. A., Burton, M. S., & McCollum, G. (2005). Effects of pre or postharvest gibberellic acid application on quality of Florida ‘Fallglo’ tangerines and ‘Ruby’ red grapefruit. Proceedings of the Florida State Horticultural Society, 118, 385-388. Recuperado de [ Links ]

Robles-González, M. M., Carrillo-Medrano, S. H., Manzanilla- Ramírez, M. A., Velázquez-Monreal, J., & Medina- Urrutia, V. M. (2010). Mejoramiento Genético de limón mexicano: Avances y perspectivas. VI Simposium Internacional Citrícola, Tecomán, Colima, México, 93- 110. Recuperado de ]

Secretaría de Agrícultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA). (2014). NMX-FF-087 SCFI-2001. Productos alimenticios no industrializados para el consumo humano-fruta fresca-limón mexicano (citrus aurantifolia Swingle).Secretaría de Economía Consultado 30-11-2014 en Secretaría de Economía Consultado 30-11-2014 en ]

Servicio de Información Agroalimentaria y Pesquera (SIAP). (2014). Consultado el 30-11-2014 en Consultado el 30-11-2014 en http://www.siap.gob.mxLinks ]

Statistical Analysis System (SAS Institute). (2002). SAS/STAT. 9.0, user’s guide. Cary. NC, USA: Author [ Links ]

Tafolla-Arellano, J. C., González-León, A., Tiznado- Hernández, M. E., Zacarías-García, L., & Báez-Sañudo, R. (2013). Composición, fisiología y biosíntesis de la cutícula en plantas. Revista Fitotecnia Mexicana, 36(1), 3-12. Recuperado de ]

Yun, Z., Gao, H., Liu, P., Liu, S., Luo, T., Jin, S., Xu, Q., Xu, J., Cheng, Y., & Deng, X. (2013). Comparative proteomic and metabolic profiling of citrus fruit with enhancement of disease resistance by postharvest heat treatment. BMC Plant Biology, 13, 44. doi: 10.1186/1471-2229-13-44 [ Links ]

Received: January 30, 2015; Accepted: January 22, 2016

Email:, tel. y fax: (595) 952 02 33 (*Corresponding author).

Creative Commons License This is an open-access article distributed under the terms of the Creative Commons Attribution License