- Similares em SciELO
versão impressa ISSN 0188-8897
Hidrobiológica vol.20 no.3 México Jan. 2010
Evidence of sexual transition in Leopard Grouper (Mycteroperca rosacea) individuals held in captivity
Evidencia de transición sexual en individuos de cabrilla sardinera (Mycteroperca rosacea) mantenidos en cautiverio
Margarita KiewekMartínez, Vicente GraciaLópez and Carmen RodríguezJaramillo
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195, Col. Playa Palo Sta. Rita, La Paz B.C.S. 23090, México. Email: firstname.lastname@example.org
Recibido: 15 de junio de 2009
Aceptado: 15 de noviembre de 2010
This study describes histological observations of the gonads of 12 captive leopard grouper, M. rosacea maintained in captivity. Monthly gonad samples during February to April 2003, were obtained by catheterization and analyzed to determine sex and degree of ovarian development. Oocytes were classified into 5 stages of development and the frequencies were obtained to describe the oocyte distribution in the ovary. Two fish that were females in February were in a bisexual stage in March and functional males in April. The transitional stage was observed during the reproductive season and included degeneration of primary oocytes and proliferation of spermatogonia.
Key words: Gonad development, leopard grouper, captivity.
Este estudio describe las observaciones histológicas del desarrollo gonadal de 12 individuos de la cabrilla sardinera, en condiciones de cautiverio. De febrero a abril del 2003 se obtuvieron muestras mensuales de la gónada mediante un catéter flexible, las cuales fueron analizadas para determinar el sexo del organismo y el estadio de desarrollo gonadal. Los ovocitos fueron clasificados en 5 etapas de desarrollo y se calcularon las frecuencias para determinar su distribución dentro del ovario. Dos individuos que fueron identificados como hembras en febrero, se encontraron en estadio bisexual en marzo y fueron machos funcionales en abril. Este estadio de transición se observó durante el periodo reproductor y se caracterizó por la degeneración de ovocitos primarios y la proliferación de espermatogonias.
Palabras clave: Cabrilla sardinera, cautiverio, desarrollo gonadal.
The leopard grouper Mycteroperca rosacea (Streets 1877) is one of five species of the genus Mycteroperca in the eastern Pacific (Rosenblatt & Zahuranec, 1967; Heemstra & Randall, 1993). M. rosacea is distributed throughout the Gulf of California as far south as the state of Jalisco, Mexico (Allen & Robertson, 1998). It inhabits rocky areas and sargasso beds near the shore and islands in depths < 50 m (GraciaLópez et al., 2004a).
Overexploitation has led to listing as a vulnerable species in the "IUCN Red List of Threatened Species" (VU A1d+2d), high risk of extinction in the wild in mediumterm future (IUCN, 2006). In the last few years, some research about the biology, natural feeding habits and ecology has been carried out (PeláezMendoza, 1997; DíazUribe et al., 2001; MendozaBustamante, 2002) being the first support for studies on reproduction technology and larval rearing (GraciaLópez et al., 2004a; GraciaLópez et al., 2004b; GraciaLópez et al., 2005; KiewekMartínez, 2004) with a possibility of repopulation of natural stocks.
Groupers belong to Serranidae family (Subfamily Epinephelinae). They mainly inhabit coastal waters in tropical, subtropical and temperate seas (AbuHakima, 1987; Brusle & Brusle, 1975; Lee et al., 2002). They primarily display protogynous hermaphroditism (Devlin & Nagahama, 2002; Lee etal., 2002; Sadovy etal., 1994), but some have been described as gonochoric where juveniles pass through a bisexual stage of gonad development like Epinephelus striatus (Bloch, 1972) (Sadovy & Colin, 1995) and most recently M. rosacea (Erisman et al., 2006). Sex change is common among marine fish. The bestknown cases have been described for labrids, serranids and sparids (Robertson & Justines, 1982; Warner, 1982), among others. It is part of some species life history and it seems to occur in one direction and in some serranid species it is socially controlled (Robertson, 1972; Ross et al., 1983).
Most studies of gonad development and sex change using histological evidence were achieved with wild sacrificed fish (AbuHakima, 1987; Bhandari et al., 2003; Erisman et al., 2008; McGovern etal., 1998; Smith, 1965). Therefore, the sequence of gonad changes taking place during the sexual transition is described based on different individuals. In fish aquaculture, sex ratio in maturation tanks is essential to obtain fertilized eggs. Therefore, sex change of individuals has notable repercussions on stock configuration and reproduction of broodstock maintained in captivity (Hong et al., 2006). Previous studies with tagged M. rosacea individuals that were maintained in captivity changed sex from female to male between breeding seasons (GraciaLópez personal communication), but there were no observations or any histological evidence of the time required to achieve complete gonad transformation or if it occurred during the breeding season or out of it. Therefore, this study was carried on to describe gonad development of leopard grouper held in captivity and to identify individuals that could change sex under these conditions.
MATERIALS AND METHODS
During June 2002, twelve leopard groupers (0.32 to 0.84 Kg) were captured by hook and line with live bait and different types of lures at San Evaristo, B.C.S. (25.9°N, 110.7°W) and transferred to research facilities (CIBNOR) located in La Paz, Mexico. Fish were anesthetized with 100 mg/L tricaine (MS222, Western Chemical), tagged in the dorsal muscle with Spaghetti Floy Tags, and weighed. Fish were maintained during 13 months in a cylindrical 1m depth and 16m3 capacity tank with filtered (< 1 µm) flowthrough seawater, supplemental aeration and natural photoperiod and temperature. Fish were fed frozen sardine, squid, or mackerel ad libitum on alternate days.
Temperature (Submersible Temperature Logger, HOBO TidbiT, WI), salinity (refractometer S/M1llE, ATAGO CO. LTD.) and dissolved oxygen (YSI 85, OH) were daily measured. Photoperiod was calculated by the method described by Rodriguez et al., (2001). Water temperature was between 25 and 29 °C (Fig. 1), salinity between 40 and 41 ppt. and dissolved oxygen 7.27.6 mg/L.
From February to July 2003 gonad samples were obtained monthly, by gonad catheterization with a polyethylene catheter inserted into the gonoduct of anaesthetized individuals (GraciaLópez et al., 2004a; 2004b). Samples were fixed for 48 h in Karnovsky fixative (20% glutaraldehyde, 40% sacarose and 40% sterilized seawater) (Karnovsky, 1965), dehydrated, embedded in paraffin, sectioned (< 5 um) and stained with Harris hematoxylineosin. Each sample was examined under an optical microscope (Olympus Bx41). Images were captured using a digital camera (CoolsnapPro color MediaCybernetics, San Diego, CA, USA) and processed with ImagePro Plus software (version 5.0, MediaCybernetics), which is designed for highresolution image analysis.
Gonads of each female were classified into 5 development stages based on the description made by Kuo etal. (1988) and Carrillo etal. (1989) for teleost fish which is based on easily recognizable morphologic characters like primary growth (stage I), early vitellogenesis (stage II), late vitellogenesis (stage III), ovulation (stage IV) and atretic follicles (stage V). Oocyte frequency in each development category was obtained from the number of oocytes in each stage in relation to the total number of analyzed oocytes (Fig. 2AH; Table 1). Fish in sexual transition or bisexual (stage VI) were identified according to the description made for Epinephelus fario and males by the observation of testis in the histological slides or the presence of sperm when gentle abdominal pressure was applied (Kuo et al., 1998).
Total length of individuals was calculated by the function y= a xb, (y = weight; a = 1.43 × 105; x = total length; b = 2.970) based on the study for wild leopard grouper, M. rosacea (DíazUribe et al., 2001).
Weight of broodstock during the study ranged from 0.34 to 1.00 Kg (Fig. 3).
Description of different ovarian stages, diameter of oocyte and germinal vesicle in each stage of development is shown in Table 1. Oocytes in primary growth were the most abundant throughout the study (6690%). In February, an increase in the percentage of oocytes at early vitellogenic stage occurred (7%) and in April, near the spawning season oocytes in primary growth decreased and an increase of the percentage of the oocytes in other stages of ovarian development was observed; the percentages of early vitellogenic, late vitellogenic, and ovulated oocytes increased to 12, 7 and 6%, respectively (Fig. 4, Table 1).
In February, ten females had oocytes in primary growth and two females had oocytes in every stage of development, including atretic oocytes. In March, the two females that had the most advanced stages of gonad development were found in sexual transition. The rest of the fish remained females. In April, there were no more transitionals individuals and this sex ratio was maintained throughout the experiment. (Table 2). In March when the bisexual fish were observed, broodstock weight was from 0.32 to 1.02 Kg. The two transitional fish weighed 0.52 and 0.86 Kg (Fig. 2).
Individuals in sexual transition had ovaries with advanced stages of development in February (Fig. 5A) characterized mainly by primary oocytes in the perinucleolar stage 74%, early vitellogenesis 9%, late vitellogenesis 2%, ovulated 1% and atretic follicles 14% (Table 3). In March, the analysis of the histological slides revealed the presence of ovotestis, gonad with both testicular and ovarian aspects (Fig. 5B). The transitional stage was characterized by the presence of oocytes in primary growth in the perinuleolar stage, followed by degenerated primary oocytes and the proliferation of spermatogonia, spermatocytes and spermatozoa with flagellum (Fig. 5 BC). At this stage, the differentiated male cysts proliferated to the interior of the lamellae as the primary oocytes degenerated and were reabsorbed.
Males were characterized by the presence of spermatogenesis. A large number of spermatogonia, spermatocytes, spermatids and spermatozoa were observed in functional testes in April (Fig. 5C).
In this study, the gonad development of the leopard grouper M. rosacea was described with the use of histological techniques. As observed in nature, broodstock had the most advanced gonad development in April (Erisman et al., 2008). Late vitellogenesis was observed in February indicating the beginning of gonad development and in April, the highest amount of oocytes in secondary growth was observed. The smallest mature individual was 3+ years (360 mm), coinciding with maturity of E tauvina (AbuHakima, 1987).
It was recently demonstrated that wild leopard grouper, M. rosacea is a gonochoric specie that goes through a juvenile inmature bisexual stage in sizes smaller than 220 mm (2 years old) (Erisman et al., 2008). In this study, the individuals that changed sex were older than 3 and 4 years, 351 and 416 mm respectively (the age was obtained based on a backward estimate (DiazUribe, 2001), and had mature ovaries with all the development stages one month prior to the sexual transition. There are some grouper species were the largest individual in a group is the one that changes sex. In other species, sex change could be observed in small size individuals like Epinephelus tauvina (Forsskal 1772) (AbuHakima, 1987), Epinephelus aeneus (Geoffroy SaintHilaire 1817) (Hassin et al., 1997) and Epinephelus merra (Bloch 1793) (Bhandari et al., 2003). However, it is usual that average female size in a population is smaller than mean size of males. This differs from M. rosacea were the size of females and males overlapped.
Individuals of M. rosacea changed sex to male eight months after their capture and the transition was complete in less than two months. These individuals had mature ovaries with every stage of development in February, later the presence of ovotestis was observed with ovarian tissue reminiscences one month previous the presence of sperm and the complete transformation to functional male in March. Captivity could influence the time for sex change, because in wild populations it is often observed out of the breeding season (Brusle & Brusle, 1975; Bhandari etal., 2003; Bhandari etal., 2004a; McGovern etal., 1998; Smith, 1965). The time required for a captive individual to change sex varies according to the specie like E. coloidesthat changed sex after 7 years of captivity (Yeh et al., 2003). In studies of captive E. merra, sexual transition was completed in a shorter period during the reproductive season which related with the high levels of sex steroids produced during this period (Alam et al., 2005; Alam et al., 2006).
In some grouper species the social unit consist of several small individuals and a large dominant male, when the male disappears or no longer can maintain control of subordinates one of the largest females could change sex assuming the male roll (Yaron & Sivan, 2005). In this study, the two sex transition fish were not the largest suggesting that captive M. rosacea has the potential to change sex in a wide size range. This has been reported in other captive grouper species like E. akaara where size of females that change sex is different to size found in wild populations (Tanaka etal., 1990) and E. striatus, which is a species described as gonochorist with the potential of sex change under natural or controlled conditions (Sadovy & Colin, 1995). There are also other species like Bostrichthys sinensis (Lacepéde, 1801) that have been characterized as gonochoric in wild populations, but in culture conditions 10 to 15 % of individuals are hermaphrodites suggesting that captivity conditions (high water temperature or chemicals introduced to rearing tanks) have an effect in sexual behavior (Hong et al., 2006).
Some grouper species like Epinephelus marginatus, E. coioides and E. merra have hormonally induced to change sex with methiltestosterone (MT), a mixture of hormones or aromatase inhibitors (Alam et al., 2006; Bhandari et al., 2004a; Bhandari et al., 2004b; Bhandari et al., 2005; Glamuzina et al., 1998; Yeh et al., 2003) to obtain a higher sperm production. Induction of adult sex reversal of E. merra with aromatase inhibitor produced males in a period of two and a half months with larger testes than in wild males, a great sperm production and high egg fertilization rate (Bhandari et al., 2004b; 2005) but the induction to sex reversal to juveniles of the same specie, produced males with small volume of sperm suggesting that sex reversal success could depend on the size and age of individuals (Candi et al., 2004; Glamuzina et al., 1998; Robertson & Justines, 1982).
In this study, the same individuals identified by their tags, were repeatedly sampled and maintained in captivity as suggested by Bhandari et al. (2003), therefore we can assure that results presented here belong to the same individual. These findings must be considered in stock configuration of broodstock for aquaculture production. Further studies on reproductive behavior of females and males in captivity along with sex reversal induction trials are needed to corroborate leopard groupers reproductive behavior in captivity.
Authors would like to thank J. Sandoval for technical assistance of broodstock in CIBNOR. This study was sponsored by Sistema de Investigación del Mar de Cortés (SIMAC), International Foundation of Science (IFS), Consejo Nacional de Pesca (CONAPESCA) and Consejo Nacional de Ciencia y Tecnología (CONACYT scholarship 172897) granted to M. Kiewek. The editing of this paper was made by Ana G. Trasvina Moreno.
AbuHakima, R. 1987. Aspects of the reproductive biology of the grouper, Epinephelus tauvina (Forskál), in Kuwaiti waters. Journal of Fish Biology 30: 213222. [ Links ]
Alam, M. S., H. Komuro, R. K. Bhandari, S. Nakamura, K. Soyano & M. Nakamura. 2005. Immunohistochemical evidence identifying the site of androgen production in the ovary of the protogynous grouper Epinephelus merra. Cell and Tissue Research 320: 323329. [ Links ]
Alam, M.A., R.K. Bhandari, Y. Kobayashi, K. Soyano & M. Nakamura. 2006. Induction of sex change within two full moons during breeding season and spawning in grouper. Aquaculture 255: 532535. [ Links ]
Allen, G.R. & D.R. Robertson. 1998. Peces del Pacífico Oriental Tropical. CONABIO, Agrupación Sierra Madre and CEMEX, México 327 p. [ Links ]
Baroiller, J.F., Y. Guiguen & A. Fostier. 1999. Endocrine and environmental aspects of sex differentiation in fish. CMLS Cellular and Molecular Life Sciences 55: 910931. [ Links ]
Bhandari, R. K., H. Komuro, S. Nakamura, M. Higa & M. Nakamura. 2003. Gonadal restructuring and correlative steroid hormone profiles during natural sex change in protogynous honeycomb grouper (Epinephelus merra). Zoological Science 20: 13991404. [ Links ]
Bhandari, R. K., H. Komuro, M. Higa & M. Nakamura. 2004a. Sex inversion of sexually immature Honeycomb Grouper (Epinephelus merra) by aromatase inhibitor. Zoological Science 21 (3): 305310. [ Links ]
Bhandari, R. K., M. Higa, S. Nakamura & M. Nakamura. 2004b. Aromatase Inhibitor induces complete sex change in the protogynous honeycomb grouper (Epinephelus merra). Molecular Reproduction and Development 67: 303307. [ Links ]
Bhandari, R. K., M. A. Alam, M. Higa, K. Soyano & M. Nakamura. 2005. Evidence that estrogen regulates the sex change of honeycomb grouper (Epinephelus merra), a protogynous hermaphrodite fish. Journal of Experimental Zoology 303 (A): 497503. [ Links ]
Bruslé, J. & S. Bruslé. 1975. Ovarian and testicular intersexuality in two protogynous Mediterranean groupers, Epinephelus aenus and Epinephelus guaza. In: Reinboth R. & SpringerVerlag (Eds.). Intersexuality in the animal kingdom, New York pp. 222227. [ Links ]
Bruslé, S. 1987. Sexinversion of the hermaphroditic protogynous teleost Coris julis L. (Labridae). Journal of Fish Biology 30 (5): 605616. [ Links ]
Candi, G., L. Castriota, F. Andaloro, M. G. Finoia & G. Marino. 2004. Reproductive cycle and sex inversion in razor fish, a protogynous labrid in the southern Mediterranean Sea. Journal of Fish Biology 64: 14981513. [ Links ]
Carrillo, M., N. Bromage, S. Zanuy, R. Serrano & F. Prat. 1989. The effect of modifications in photoperiod on spawning time, ovarian development and egg quality in the sea bass (Dicentrarchus labrax L.). Aquaculture 81: 351365. [ Links ]
Coleman, F., C. Koenig & L. A. Collins. 1996. Reproductive styles of shallowwater groupers (Pisces: Serranidae) in the eastern Gulf of Mexico, consequences of fishing spawning aggregations. Environmental Biology of Fish 47: 129141. [ Links ]
Devlin, R. H. & Y. Nagahama. 2002. Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Review article. Aquaculture 208: 191364. [ Links ]
DiazUribe, J. G., J. F. ElorduyGaray & M. T. GonzalezValdovinos. 2001. Age and growth of the leopard grouper, Mycteroperca rosacea, in the southern gulf of California, Mexico. Pacific Science 55: 171182. [ Links ]
Erisman, B. E., J. A. RosalesCasián & A. Hastings. 2008. Evidence of gonochorism in a grouper, Mycteroperca rosacea from the Gulf of California, Mexico. Environmental Biology of Fish 15735133 (Online). doi:10.1007/s1064100792461. [ Links ]
Glamuzina, B., N. Glavic, B. Skaramuca & V. Kozul. 1998. Induced sex reversal of dusky grouper, Epinephelus marginatus (Lowe). Aquaculture Research 29: 563567. [ Links ]
GraciaLópez, V., J. RodríguezRomero & J. M. PérezRamírez. 2004a. Induced spawning and embryonic development of the leopard grouper Mycteroperca rosacea (Streets, 1877). Ciencias Marinas 30 (2): 279284. [ Links ]
GraciaLópez, V., M. KiewekMartínez & M. MaldonadoGarcía. 2004b. Effects of temperature and salinity on artificially reproduced eggs and larvae of leopard grouper Mycteroperca rosacea. Aquaculture 237: 485498. [ Links ]
GraciaLópez, V., M. KiewekMartínez, M. MaldonadoGarcía, P. MonsalvoSpencer, G. PortilloClark, R. CiveraCerecedo, M. LinaresAranda, M. RoblesMungaray & J. M. MazónSuástegui. 2005. Larvae and juvenile production of the leopard grouper, Mycteroperca rosacea (Streets, 1877). Aquaculture Research 36 (1): 110112. [ Links ]
Hassin, S., D. Monbrison, Y. Hanin, A. Elizur, Y. Zohar & D. M. Popper. 1997. Domestication of the white grouper, E. aeneus: 1. Growth and reproduction. Aquaculture 156 (34): 305316. [ Links ]
Heemstra, P. C. & J. E. Randall. 1993. FAO species catalogue. Groupers of the world (Family Serranidae, Subfamily Epinephelinae). FAO Fish Synopsis 125 (16): 1382. [ Links ]
Hong W. S., S. X. Chen, W. Y. Zheng, Y. Xiao & Q. Y. Zhang. 2006. Hermaphroditism in cultured Chinese black sleeper (Bostrichthys sinensis L.). Journal of the World Aquaculture Society 37 (4): 363369. [ Links ]
Johnson, A. K., P. Thomas & R. R. Jr. Wilson. 1998. Seasonal cycles of gonadal development and plasma sex steroid levels in Epinephelus morio, a protogynous grouper in the eastern Gulf of Mexico. Journal of Fish Biology 52: 502518. [ Links ]
Karnovsky, M. J. 1965. A formaldehydeglutaraldehyde fixative of high osmolarity for use in electron microscopy. Journal of Cell Biology 27 A: 137138. [ Links ]
KiewekMartínez, N. M. 2004. Contribución al conocimiento de la reproducción en cautiverio de la cabrilla sardinera Mycteroperca rosacea. Tesis de Maestría en Ciencias (Biología) CIBNOR, Mexico. 107 pp. [ Links ]
Lee, Y. D., S. Ho Park, A. Takemura & K. Takano. 2002. Histological observations of seasonal reproductive and lunarrelated spawning cycles in the female honeycomb grouper Epinephelus merra in Okinawan waters. Fisheries Science 68 (4): 872877. [ Links ]
McGovern, J. C., D. M. Wyanski, 0. Pashuk, C. S. II Manooch & G. R. Sedberry. 1998. Changes in the sex ratio and size at maturity of gag, Mycteroperca microlepis, from the Atlantic coast of the southeastern United States during 19761995. Fisheries Bulletin 96: 797807. [ Links ]
MendozaBustamante, J. A. 2002. Aspectos ecológicos del reclutamiento de la cabrilla sardinera Mycteroperca rosacea (Streets, 1877) (Pisces: Serranidae) en camas de sargazo, San Juan de la Costa, B.C.S., México. Tesis de Licenciatura (Biología) UABCS, Mexico. [ Links ]
Nakamura, M., T. F. Hourigan, K. Yamauchi, Y. Nagahama & E. G. Grau. 1989. Histological and ultrastructural evidence for the role of gonadal steroid hormones in sex change in the protogynous wrasse (Thalassoma duperrey). Environmental Biology of Fishes 24: 117136. [ Links ]
PeláezMendoza, A. K. 1997 Hábitos alimenticios de la cabrilla sardinera Mycteroperca rosacea Streets, 1877 (Pises: Serranidae) en la bahía de La Paz B.C.S y zonas adyacentes. Tesis de Licenciatura (Biología) UABCS, Mexico. 62 p. [ Links ]
Quinitio G.F., N. B. Caberoy & D. M. Jr Reyes. 1997. Induction of sex change in female Epinephelus coioides by social control. Israeli Journal of Aquaculture Bamidgeh 49: 7783. [ Links ]
Robertson, D. R. 1972. Social control of sex reversal in a coralreef fish. Science 173: 10071009. [ Links ]
Robertson, D. R. & G. Justines. 1982. Protogynous hermaphroditism and gonochorism in four Caribbean reef gobies. Environmental Biology of Fishes 7 (2): 137142. [ Links ]
Rodríguez, L., I. Begtashi, S. Zanuy, M. Shaw & M. Carrillo. 2001. Changes in plasma levels of reproductive hormones during first sexual maturation in European male sea bass (Dicentrarchus labrax L.) under artificial day lengths. Aquaculture 202 (34): 235248. [ Links ]
Rosenblatt, R. H. & B. J. Zahuranec. 1967. The Eastern Pacific grouper of the genus Mycteroperca, including a new species. California Fish and Game 53: 228245. [ Links ]
Ross, R. M., G. S. Losey & M. Diamond. 1983. Sex change in a coralreef fish: dependence of stimulation and inhibition on relative size. Science 221: 574575. [ Links ]
Sadovy, Y., P. L. Colin & M. L. Domier. 1994. Aggregation and spawning in the tiger grouper Mycteroperca tigris (Pisces: Serranidae). Copeia 2: 511516. [ Links ]
Sadovy, Y. & P. L. Colin. 1995. Sexual development and sexuality in the Nassau grouper. Journal of Fish Biology 46: 961976. [ Links ]
Shapiro, D. Y. 1980. Serial female sex changes after simultaneous removal of males from social groups of coral reef fish. Science 209: 11361137. [ Links ]
Shapiro, D. Y. 1987. Differentiation and evolution of sex change in fishes. BioScience 37: 490497. [ Links ]
Smith, C. L. 1965. The pattern of sexuality and the classification of serranid fishes. American Museum Novitat2207: 120. [ Links ]
Tanaka, H., K. Hirose, K. Y. Nogami, K. Hattori & N. Ishibashi. 1990. Sexual maturation and sex reversal in red spotted grouper, Epinephelus akaara. Bulletin of Natural Research Institute of Aquaculture 17: 115. [ Links ]
Warner, R. R. 1982. Mating systems, sex change and sexual demography in the rainbow wrasse, Thalassoma lucasanum. Copeia 3: 653661. [ Links ]
Yaron, Z. & B. Sivan. 2005. Reproduction. In: Evans D.H. & J.B. Clairborne (Eds.). The physiology of fishes. Third edition, CRC Press, Boca Raton, Florida pp. 343386. [ Links ]
Yeh, S. L., C. M. Kuo, Y. Y. Ting & C. F. Chang. 2003. Androgens stimulate sex change in protogynous grouper, Epinephelus coioides: spawning performance in sexchanged males. Comparative Biochemistry and Physiology 135 C: 37538. [ Links ]