CONCENTRACIONES DE ELEMENTOS TRAZA EN ALGUNOS ACEITES COMESTIBLES DE RIAD

Alrajhi, Ibrahim Mohammed; Idriss, Hajo; Alrajhi, Ibrahim Mohammed; Idriss, Hajo

doi:10.20937/rica.53562

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Revista internacional de contaminación ambiental

versão impressa ISSN 0188-4999

Rev. Int. Contam. Ambient vol.36 no.4 Ciudad de México Nov. 2020 Epub 13-Set-2021

https://doi.org/10.20937/rica.53562

Artículos

CONCENTRACIONES DE ELEMENTOS TRAZA EN ALGUNOS ACEITES COMESTIBLES DE RIAD

CONCENTRATION OF TRACE METALS IN SOME MAJOR EDIBLE OILS OF RIYADH

Ibrahim Mohammed Alrajhi¹

Hajo Idriss²³^*

^¹Prince Sultan Military Medical City, Po. Box 7897 Riyadh 11159, Kingdom of Saudi Arabia.

^²Deanship of Scientific Research, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia

^³Sudan Atomic Energy Commission.

RESUMEN

Los elementos químicos desempeñan un papel importante en varios procesos metabólicos en el cuerpo humano. Además, algunos de estos elementos son tóxicos si se consumen en cantidades excesivas. En esta investigación se estudió un total de 11 elementos (Mn, Fe, Cu, Cr, Zn, Ni, Al, Pb, As, Se y Al) en muestras de aceite vegetal comestible mediante el uso de un espectrómetro de emisión óptica de plasma acoplado inductivamente. Se encontró que la concentración de los metales estaba en un nivel de traza inferior al límite de concentración máximo establecido por la Organización Mundial de la Salud, así como de la ingesta diaria recomendada de esos metales. Los coeficientes de correlación de Pearson mostraron una fuerte correlación entre Cr y Fe (0.998), Cr y Mn (0.994), Cr y Ni (0.985), Fe y Mn (0.994), Fe y Ni (0.985), Mn y Ni (0.978) y As y Se (0.997). Se puede concluir que las muestras de aceite vegetal comestible estaban libres de contaminación por elementos químicos.

Palabras clave: aceite vegetal comestible; metales pesados; elementos esenciales; ICP-OES; RAS

ABSTRACT

Chemical elements play major roles in various metabolic processes in the human body. Moreover, some of these elements are toxic if consumed in excessive quantities. In this study, a total of 11 elements (Mn⁺², Fe⁺², Cu⁺², Cr⁺³, Zn⁺², Ni⁺², Se, Pb⁺², As⁺³, and Al⁺³) were studied in edible vegetable oil samples by using an inductively coupled plasma-optical emission spectrometer. The concentration of the metals was found to be in trace level lower than the maximum allowable concentration limit set by the World Health Organization as well as the recommended daily intake of metals. Pearson correlation coefficients showed strong correlations between Cr and Fe (0.998), Cr and Mn (0.994), Cr and Ni (0.985), Fe and Mn (0.994), Fe and Ni (0.985), Mn and Ni (0.978), and As and Se (0.997). It can be concluded that the edible vegetable oil samples were free of any chemical element contamination.

Key words: vegetable edible oil; heavy metals; essential elements; ICP-OES; KSA

INTRODUCTION

Edible oils are extremely important food for all people around the world. They are extracted from plants (e.g., soybean, canola, and chili); seeds (e.g., corn, sesame and sunflower); nuts (e.g., walnut and macadamia); and fruits (e.g., watermelon palm, olive, and coconut). Relying on oil sort, they are widely used in cooking (bread preparation and frying food) and for non-cooking products such as salad, pastry, and appetizers. In addition, edible oils are used to produce nonfood products such as cosmetics, pharmaceutical, and as biofuels.

The human body utilizes oils and fats in the diet for important purposes, as energy and vitamins suppliers, and also as essential elements in metabolic reactions in the human body (^{Dugo et al. 2004}). Human beings, plants and animals rely on major metals such as Ca, Mg, Fe, Cu, Zn and Mn for growth and development, though some metals are benefic and others can be toxic, even in low quantity, and thus pose health risks to humans and animals (^{Sanches-Filho et al. 2017}). While the effect of chronic exposure to small amounts of some metals seems to be well understood, several incidents show the seriousness of high levels of exposure to some noxious metals, particularly Cd, Cr⁺³, and Pb (^{Garrido et
al. 1994}, ^{Buldini et al. 1997}, ^{Rajeshkumar et al. 2017}). The quality of edible oils is directly linked to the concentration of many trace metals. Concentrations of Fe, Cu, Co, Ni and Mn are known to increase the rate of oil oxidation, while elements like As, Cr, Cd, and Pb are significant on account of their toxicity and metabolic role (^{Manjusha, et al.
2019}).

The presence of metals in vegetable edible oils relies on many factors. They may come from the soil, environment, and genetic structure of the plant, as well as fertilizers and pesticides introduced during the production process or by contamination from the metal processing equipment or shipping containers (^{Cindiric et al. 2007}, ^{Jamali et al. 2008}). If toxic metals are consumed the risk of a particular population may increase, so it is essential to determine the concentration of these metals (^{Sobhan 2016}). Many studies have been recently carried out on the determination of heavy metals in edible oils all over the world (^{Cindiric et al.
2007}, ^{Mendil et al. 2009}, ^{Bakkali et al. 2012}, ^{Savio et al. 2014}, ^{Barreto et
al. 2018}, ^{Manjusha et al. 2019}, ^{Karasakal 2020}). Food contamination by heavy metals has become a reality and constitute a challenge for many countries (^{Karimi et al. 2015}). The purpose of this work is to determine the concentrations of essential and potentially toxic metals in edible vegetable oils sold in Riyadh markets.

MATERIALS AND METHODS

Study area

The study area was Riyadh, one of the biggest cities and capital of the Kingdom of Saudi Arabia, with an area of about 1554 km² and an estimated population of 7 231 447. It is located centrally in the Najd region and lies between latitude 34º-38º N and longitude 43º- 46º E, as shown in figure 1.

Fig. 1 Map of Saudi Arabia showing Riyadh city.

Sample preparation

A total of 54 edible vegetable oil samples (soybean, palm and olive) were collected from the supermarkets around the city of Riyadh and transported to the environmental research laboratory at Imam Mahammad Ibn Saud University for elemental analysis. Ten milliliters of high purity HNO₃ 65 % and 2 ml of H₂O₂ 30 % were added to the beaker containing 2 g of dry samples and were placed in the fume cupboard for two days for digestion. The mixture was then digested at 80 ºC till a transparent solution was achieved. After cooling, the digested samples were filtered using Buchner funnels and the filtrate was diluted to 45 mL with distilled water. Then the samples were analyzed using inductively coupled plasma atomic emission spectroscopy (ICP-AES). The analyzed elements were Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn, As, Se and Al (^{al-Rajhi 2014}).

Spectrometric analysis

The measurements were performed using a Genesis ICP optical emission spectrometer (Spectro Analytical Instruments, Kleve, Germany) with axial plasma observation. The instrument includes a Paschen-Runge mount spectrometer, constructed employing the optimized Rowland Circle Alignment (ORCA) technique. It consists of two hollow section cast shells, designed for direct thermal stabilization and small volume. Fifteen pre-aligned linear coupled-charge devices (CCDs) were installed outside the optics body, which allows fast, simultaneous spectrum capture of the wavelength range between 175 and 777 nm. For UV access < 200 nm, the optical system was purged with argon at a rate of 0.5 L/min during normal operation. Intelligent calibration logic (ICAL) was used to normalize the wavelength and the intensity of the optical system to a reference optic (optic master). The stability of the forward power in the case of rapidly changing sample loads was achieved by using an air-cooled ICP-generator based on a free running 27.12 MHz system. All ICP operating parameters were software controlled. The ICP-OES instrument was initialized and allowed to achieve thermal equilibrium over 30 min. ICP-OES determinations of elements concentration were performed using the emission lines 228.802, 267.716, 324.752, 238.204, 285.213, 259.372, 231.604, 220.353, 213.857, 189.641, 196.090, and 384.401 λ (nm) for the elements Cd, Cr, Cu, Fe, Mg, Mn, Ni, Pb, Zn, As, Se, and Al, respectively (^{al-Rajhi 2014}).

Quality control

All the standard stock solutions of heavy metals were certified reference materials purchased from Agilent Technologies (USA). HNO₃ and H₂O₂ were heavy metal analytical grade purchased from Wako Chemical (USA). Reagent water, toluene and acetone were of analytical reagent grade purchased from J.T. Baker (USA). Adequate quality control method and vigilance were carried out in order to get reliable results. Throughout the experiments, all glassware and equipment were cautiously washed beginning with acetone followed by 5 % HNO₃ and ending with repeated rinsing distilled water to prevent contamination. Reagent blank determinations were used to correct the readings. The lower detection limit values of the elements were found in this study to be 0.001 mg/kg for Cr, 0.001 mg/kg for Cu, 0.0001 mg/kg for Zn, 0.002 mg/kg for Fe, 0.003 mg/kg for Mn, 0.0001 mg/kg for Cd, 0.001 mg/kg for Al, 0.001 mg/kg for Ni, 0.01 mg/kg for Se, 0.004 for Pb and 0.01 mg/kg for As. The recovery values were nearly quantitative (95%) for digestion method. The relative standard deviations were less than 10 % for all investigated elements. Multi-element solution standards obtained from Agilent Technology were used to calibrate and calculate sample results.

RESULTS AND DISCUSSION

The determination of the elemental concentration in edible vegetable oil is vital, since several elements play major roles in various metabolic processes in the human body. Moreover, some of these elements are toxic if consumed in excessive quantities. In this study, 11 elements (Mn, Fe, Cu, Cr, Zn, Ni, Al, Pb, As, Cd, Se, and Al) in edible vegetable oil samples were studied using ICP-OES, as shown in table I and Fig. 2.

TABLE I AVERAGE CONCENTRATIONS* OF Cd, Cr, Cu, Fe, Mn, Ni, Pb, Zn, As, Se AND Al IN EDIBLE VEGETABLE OIL.

No.	Cd	Cr	Cu	Fe	Mn	Ni	Zn	Al	Pb	As	Se
1	0.001	0.004	0.004	0.061	LDL	0.038	0.013	0.179	0.006	0.28	0.278
2	LDL	0.009	0.005	0.048	0.001	0.042	0.004	0.014	0.004	0.099	0.102
3	0.001	0.014	0.005	0.305	0.001	0.018	0.013	0.295	0.004	0.309	0.284
4	0	0.004	0.007	0.056	0.001	0.026	0.048	0.111	0.004	0.18	0.183
5	0.001	0.009	0.009	0.239	0.002	0.222	0.059	0.131	0.086	0.877	0.824
6	LDL	0.009	0.005	0.067	0.001	0.085	0.027	0.069	0.011	0.24	0.238
7	LDL	0.004	0.005	0.12	0.001	0.08	0.017	0.425	0.004	0.289	0.3
8	LDL	0.004	0.005	0.072	0.001	0.052	0.01	0.116	0.004	0.309	0.305
9	LDL	0.01	0.006	0.106	0.001	0.011	0.014	0.152	0.004	0.256	0.242
10	LDL	0.008	0.019	0.108	0.003	0.018	0.049	0.087	0.004	0.026	0.064
11	LDL	0.008	0.004	0.137	0.001	0.01	0.023	0.149	0.004	0.331	0.342
12	0.001	0.007	0.005	0.084	0.001	0.013	0.024	0.098	0.032	0.614	0.587
13	LDL	0.008	0.005	0.067	0.001	0.018	0.023	0.083	0.004	0.381	0.377
14	0.001	0.007	0.007	0.084	0.001	0.009	0.014	0.098	0.046	0.975	0.89
15	LDL	0.004	0.005	0.372	0.003	0.014	0.022	0.079	0.008	0.364	0.343
16	LDL	0.002	0.004	0.027	LDL	0.034	0.02	0.019	0.01	0.321	0.298
17	LDL	0.01	0.005	0.038	0.002	0.032	0.021	0.013	0.004	0.081	0.085
18	LDL	0.003	0.004	0.028	LDL	0.052	0.001	0.012	0.004	0.058	0.059
19	LDL	0.011	0.005	0.065	0.001	0.046	0.01	0.091	0.004	0.337	0.322
20	LDL	0.004	0.005	0.049	LDL	0.026	0.007	0.081	0.004	0.254	0.242
21	LDL	0.007	0.005	0.089	0.001	0.007	0.01	0.089	0.004	0.395	0.393
22	LDL	0.004	0.004	0.066	LDL	0.006	0.007	LDL	LDL	LDL	LDL
23	0.002	1.211	0.018	4.916	0.077	0.785	0.234	LDL	LDL	LDL	LDL
24	LDL	0.018	0.011	0.112	0.001	0.042	0.155	LDL	LDL	LDL	LDL
25	LDL	0.004	0.002	0.025	LDL	0.002	0.003	LDL	LDL	LDL	LDL
26	LDL	0.005	0.004	0.079	LDL	0.007	0.014	LDL	LDL	LDL	LDL
27	0.001	1.898	0.032	7.861	0.128	1.215	0.018	LDL	LDL	LDL	LDL
28	0.011	0.022	0.001	0.173	0.002	0.012	0.004	LDL	LDL	LDL	LDL
29	0.002	0.003	0.001	0.034	0	0.004	0.007	LDL	LDL	LDL	LDL
30	LDL	0.012	0.006	0.151	0.001	0.015	0.008	LDL	LDL	LDL	LDL
31	LDL	0.013	0.004	0.068	0.001	0.009	0.015	LDL	LDL	LDL	LDL
32	0.007	0.002	0.001	0.034	LDL	0.002	0.062	LDL	LDL	LDL	LDL
33	LDL	0.003	0.005	0.041	LDL	0.006	0.01	LDL	LDL	LDL	LDL
34	LDL	0.014	0.046	0.141	0.002	0.016	0.028	LDL	LDL	LDL	LDL
35	LDL	0.004	0.005	0.049	LDL	0.01	0.01	LDL	LDL	LDL	LDL
36	LDL	0.005	0.004	0.055	0.001	0.008	0.018	LDL	LDL	LDL	LDL
37	LDL	0.007	0.009	0.088	0.001	0.016	0.016	LDL	LDL	LDL	LDL
38	LDL	0.006	0.006	0.074	0.001	0.012	0.031	LDL	LDL	LDL	LDL
39	0.001	0.038	0.007	0.278	0.005	0.065	0.089	0.074	0.087	0.799	0.778
40	0.002	0.002	0.004	0.033	0.001	0.014	0.07	0.017	0.033	0.302	0.317
41	LDL	0.062	0.032	0.252	0.003	0.035	0.041	0.059	0.008	0.107	0.142
42	LDL	0.003	0.005	0.026	0.001	0.004	0.035	0.021	0.016	0.147	0.165
43	0.001	0.025	0.007	0.356	0.003	0.024	0.084	0.031	0.021	0.109	0.136
44	LDL	0.035	0.008	0.133	0.003	0.028	0.048	0.094	0.017	0.134	0.15
45	LDL	0.012	0.008	0.094	0.001	0.01	0.055	0.024	0.019	0.121	0.128
46	LDL	0.024	0.024	0.174	0.003	0.088	0.511	0.199	0.015	0.112	0.134
47	LDL	0.003	0.005	0.049	0.002	0.03	0.086	0.012	0.021	0.074	0.086
48	LDL	0.004	0.005	0.08	0.001	0.01	0.037	0.013	0.018	0.081	0.088
49	LDL	0.003	0.007	0.038	LDL	0.004	0.05	0.013	0.017	0.083	0.092
50	LDL	0.01	0.022	0.08	0.005	0.017	0.067	0.078	0.006	0.045	0.082
51	LDL	0.036	0.012	0.181	0.003	0.03	0.079	0.095	0.013	0.092	0.111
52	LDL	0.019	0.081	0.142	0.001	0.02	0.051	0.074	0.004	0.01	0.051
53	LDL	0.049	0.02	0.18	0.018	0.024	0.033	0.063	0.004	0.01	0.05
54	LDL	0.078	0.027	0.633	0.006	0.057	0.062	0.08	0.011	0.031	0.07
Min	0.001	0.002	0.001	0.025	0.001	0.002	0.001	0.012	0.004	0.01	0.05
Max	0.011	1.898	0.081	7.861	0.128	1.215	0.511	0.425	0.087	0.975	0.89
Avg	0.0006	0.072	0.011	0.362	0.006	0.065	0.047	0.06	0.011	0.17	0.172
Std	0.002	0.307	0.013	1.256	0.02	0.196	0.077	0.073	0.018	0.229	0.215

*In mg/kg.

LDL: lower detection limit, Min: minimum, Max: maximum, Avg: average, Std: standard deviation.

Fig. 2 Cr, Cu, Fe, Mn, Ni, Zn, Al, Pb, As, As, and Se concentrations (in mg/kg) and statistical summary of edible vegetable oil samples.

Nickel

Ni concentrations varied from 0.002 to 1.215 mg/kg with an average value of 0.065 mg/kg. The concentration of Ni control samples was 0.01 mg/kg. Upon comparing the average values of Ni in the obtained results with control and global data, it was found that the obtained values were far below the reported average values from Pakistan, Turkey and Spain (^{Cindiric et al.
2007}, ^{Lorent et al. 2011}). The nickel average daily intake (0.023011 mg/kg) was found to be lower than the maximum allowable concentration limit set by the World Health Organization (100-300 mg/kg) (^{WHO 1995}). Trace amounts of Ni may be beneficial as activators of some enzyme systems, but its toxicity at higher levels is more prominent (^{Ameen et al.
2019}).

Copper

Cu concentrations ranged from 0.001-0.081 mg/kg with an average value of 0.011 mg/kg. The concentration of control samples was 0.0001 mg/kg. The Cu concentration range was lower than that reported in similar data (0.02-0.33 mg/kg) (^{Garrido et al. 1994}), 12.71-50.5 mg/kg (^{Buldini et al. 1997}), 0.15-1.5 mg/kg (^{Zeiner et al. 2005}), 0.0184-0.2870 mg/kg, 0.05-0.71 mg/kg (^{Mendil et al.
2009}), and 0-130 mg/kg (^{Llorent et
al. 2011}). The Cu average daily intake (0.004 mg/kg) was found to be lower than the maximum allowable concentration limit set by the Food and Agriculture Organization of the United Nations and WHO (3 mg/kg) (^{FAO-WHO 1999}). Cu has numerous functions in the human body, but excessive intake of this element has been reported to be toxic.

Zinc

Zn concentrations varied from 0.001 to 0.511 mg/kg ith an average value of 0.047 mg/kg. From the obtained results, the Zn concentration range was higher than the control samples (0.002 mg/kg), however Zn concentrations in this investigation were lower than in similar data reported by numerous researchers (^{Garrido et al. 1994}, ^{Mendil et al. 2009}). The Zn average daily intake (0.017 mg/kg) was found to be lower than the maximum legal limit set by ^{WHO (1995)} (60 mg mg/kg). Zn has an essential function in carbohydrate and cholesterol metabolism.

Manganese

Mn is essential in many biological processes such as immunity functions, blood sugar regulation and bone growth, as well as being a cell antioxidant (^{Pfalzer and Bowman 2017}). This element was detected in 43 of the 54 samples within a range of 0.001-0.128 mg/kg with an average value of 0.002 mg/kg. In comparison, manganese average values in the investigated samples were lower than in a similar studies (^{Garrido et al. 1994}, ^{Cindiric et al. 2007}, ^{Mendil et al.
2009}, ^{Llorent et al. 2011}). Manganese average daily intake 0.006 mg/kg was found to be lower than the maximum limit set by ^{WHO (1995)} (2.5-5 mg/kg).

Chromium

Cr is important in the metabolism of glucose, as reported by ^{Yagi et al. (2013)}. The toxic effects of Cr⁺³ intake include skin rash, nose irritation, bleeding, upset stomach, kidney and liver damage. Cr concentrations varied from 0.002 to 1.898 mg/kg with an average value of 0.026 mg/kg. The concentration of control samples was 0.003 mg/kg.

Iron

Fe was found within the range of 0.025-7.861 mg/kg with an average value of 0.362 mg/kg. The average daily intake of this element (0.129 mg/kg) was found to be lower than the maximum allowable limit (11-18 mg/kg) (Institute of Medicine 2001). The concentrations of control samples was 0.027 mg/kg. It is well known that Fe is indispensable for human beings and animals and is an essential component of hemoglobin. It facilitates the oxidation of carbohydrates, proteins and fats to control body weight, which is an extremely important factor in diabetes; furthermore, an Fe deficiency can induce anemia. The recommended daily allowance (RDA) for Fe is 11 and 18 mg/day for children and adults, respectively (^{Schüman et al. 2007}).

Selenium

Se was present in a range of 0.05-0.89 mg/kg with an average of 0.172 mg/kg, while the control sample concentration was 0.004 mg/kg. The average daily intake of Se (0.025) mg was found to be lower than the maximum allowable concentration limit 0.55 mg/kg (^{Schüman et al. 2007}) (^{Institute of Medicine 2001}). Se is an essential element for human nutrition and plays an important role in the protection of the body from cancer and heart disease (^{Wan et al. 2020}). Its RDA is 0.55 mg/day (^{Institute of Medicine 2001}, ^{Schüman et al. 2007}).

Cadmium

Ca was detected in 14 of the 54 samples. Concentrations of this element in the samples were found to be in the range of 0.001-0.011 mg/kg, with an average value of 0.0006 mg/kg. The concentration in control samples was 0.001 mg/kg. All the investigated samples showed lower Ca levels than the legal limits recommended by FAO-WHO (0.5 mg/kg) (^{Codex S
2011}). Ca is an extremely toxic metal found naturally in soil, but also spread in the environment due to human activity. Overexposure to Ca can lead to lung, liver, skeletal, and renal problems, as well as cancer (^{Djordjevic et al. 2019}).

Arsenic

The As content in samples were in the range of 0.01-0.975 mg/kg with an average value of 0.17 mg/kg. The obtained values fell far below the action level recommended by FAO/WHO of 0.5 mg/kg (^{Codex S
2011}). The control sample concentration was 0.01mg/kg. As is a naturally occurring element that can be toxic to humans, animals and plants; however, its toxicity varies depending on its presentation form (^{Kim et al. 2018}).

Aluminum

Al concentrations were in the range of 0.012-0.425 mg/kg, with an average value of 0.06 mg/kg. The control sample concentration was 0.001 mg/kg. The toxicity of Al in humans is mainly related to neurotoxicity and the development of neurodegenerative diseases (^{Verstraeten et al.
2008}). It was difficult to compare our obtained results for Al with worldwide concentrations due to the scarcity of data. The FAO-WHO tolerable weekly intake value for Al is 1 mg/kg body weight/week (^{Stahl et al. 2011}).

Lead

Pb was detected in 16 of the 37 samples with concentrations ranging from 0.004 to 0.087 mg/kg and an average of 0.011 mg/kg. The Pb control sample concentration was 0.01 mg/kg. From the obtained results, the Pb concentration range was lower than in similar data reported in Turkey. The Pb concentration in the current study fell within the recommended limits set by FAO/WHO (0.1 mg/kg) (^{Codex S 2011}). Lead is a naturally occurring element and is a widespread industrial metal. Pb has severe health effects even at relatively low levels. It is able to cross the placenta and damage the developing fetal nervous systems (^{Łukomska et
al. 2017}). Pb causes both acute and chronic poisoning; it has adverse effects on the kidneys, liver, heart and both the vascular and immune systems.

Correlation coefficient test

Correlations support the interpretation of meaningful measurements. This approach also helps to identify common factors, inducing the observation of elemental relationships. A high value of the coefficient reveals a high linear correlation between the contents of two elements. Table II shows the Spearman correlation coefficient (r) between the elements in oil samples. It was found that the correlation matrix of investigated oil samples showed a cluster of high positive correlation between the variables Cr and Fe (0.998), Cr and Mn (0.994), Cr and Ni (0.985), Fe and Mn (0.994), Fe and Ni (0.985), Mn and Ni (0.978), As and Se (0.997). This indicates that there is a strong relationship between these elements. On the other hand, moderate positive correlations were noted between Pb and As (0.768), and between PB and Se (0.778). Overall, the correlation behavior of elements in the studied samples remained noticeably diverse, which may be attributed to the disproportions of the origin of samples.

TABLE II PEARSON CORRELATION COEFFICIENTS FOR Mn, Fe, Cu, Cr, Zn, Ni, Al, Pb, As, Se AND Al IN EDIBLE VEGETABLE OIL SAMPLES.

	Cd	Cr	Cu	Fe	Mn	Ni	Zn	Al	Pb	As	Se
Cd	1.000
Cr	0.085	1.000
Cu	-0.150	0.256	1.000
Fe	0.087	0.998	0.260	1.000
Mn	0.076	0.994	0.269	0.992	1.000
Ni	0.070	0.985	0.243	0.985	0.978	1.000
Zn	-0.007	0.154	0.228	0.154	0.152	0.191	1.000
Al	-0.136	-0.145	0.008	-0.125	-0.137	-0.080	0.136	1.000
Pb	0.008	-0.106	-0.072	-0.090	-0.094	-0.011	0.145	0.143	1.000
As	-0.042	-0.149	-0.208	-0.133	-0.147	-0.066	-0.062	0.433	0.768	1.000
Se	-0.056	-0.159	-0.180	-0.142	-0.154	-0.076	-0.044	0.454	0.778	0.997	1.000

Note: values in bold characters represent a > 0.5 correlation.

CONCLUSIONS

Based on the obtained results the following conclusions may be drawn:

No contamination was observed in the samples analyzed.
There are no significant differences in the concentrations of studied oils because all of them were found to be lower than the maximum allowable limits set by FAO and WHO.
Significant correlations were observed between Cr and Fe (0.998), Cr and Mn (0.994), Cr and Ni (0.985), Fe and Mn (0.994), Fe and Ni (0.985), Mn and Ni (0.978), and As and Se (0.997)

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Recibido: 01 de Marzo de 2019; Aprobado: 01 de Marzo de 2020

^* Corresponding author: hjoidriss@gmail.com

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