SciELO - Scientific Electronic Library Online

 
vol.36 issue3Induction of defense response in tomato plants against Forl by garlic extractAntifungal property of honey on in vitro development of Colletotrichum gloeosporioides author indexsubject indexsearch form
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • Have no similar articlesSimilars in SciELO

Share


Revista mexicana de fitopatología

On-line version ISSN 2007-8080Print version ISSN 0185-3309

Rev. mex. fitopatol vol.36 n.3 Texcoco Oct./Dec. 2018

http://dx.doi.org/10.18781/r.mex.fit.1806-1 

Phytopathological notes

Microorganisms associated with alfalfa crown rot in north central Mexico

Rodolfo Velásquez-Valle1 

Luis Roberto Reveles-Torres1  * 

Hugo Talavera-Correa2 

1 Campo Experimental Zacatecas, INIFAP. Km. 24.5 Carretera Zacatecas - Fresnillo, C.P. 98500, Calera de V. R., Zacatecas

2 Unidad Académica de Biología, Universidad Autónoma de Zacatecas.

Abstract:

Alfalfa is an important forage crop in the states of Aguascalientes, Durango, and Zacatecas located in the northern-center of Mexico. Very scarce information is available on the spots of killed plants attributed to the disease known as crown rot and its associated microorganisms in this region. Therefore, the objective of the current work was to identify the genera of microorganisms associated to crown rot in the mentioned states. Samples of alfalfa plants showing crown rot symptoms and soil around them were collected in 30 alfalfa fields. Thirteen nematodes genera were identified in the soil samples: (Aphelenchus, Aphelenchoides, Criconemoides, Ditylenchus, Dorylaimus, Helicotylenchus, Mononchus, Pratylenchus, Psilenchus, Rhabditis, Trichodorus, Tylenchus, and Xiphinema) as well as five fungi (Fusarium, Rhizoctonia, Verticillium) and oomycetes (Phytophthora, and Pythium) genera in the crown tissues. It is remarkable the high incidence of Ditylenchus (56.7%), Pratylenchus (50%) and Fusarium fungi (100%).

Key words: fungi; nematodes; oomycetes; regional distribution

Alfalfa (Medicago sativa) is a perennial crop that accounts for 27% of national forage production. In Mexico, the area sown to alfalfa in 2018 was a bit more than 387,000 hectares with an average yield of green forage of 58.7 t ha-1 year-1 (SIAP, 2018). The states with the highest alfalfa production at the national level are Chihuahua, Guanajuato, Hidalgo, Baja California, Sonora, Durango, Coahuila and Puebla, which together account for 70% of national production (Lara and Jurado, 2014). Alfalfa is an important crop in north-central Mexico, where the states of Aguascalientes, Durango (Los Llanos) and Zacatecas are located. Large variable-sized spots of dead alfalfa plants have been observed in most commercial plots, but there is little or no information about the organisms that cause crown rot or the extent of losses caused by the disease. Alfalfa crown rot is spread throughout the world. Several Fusarium species are among the fungi most commonly isolated from necrotic crowns and roots of alfalfa plants (Uddin and Knous, 1991); in Sudan, Ao et al. (2018) mention that Fusarium, Rhizoctonia and some Pythium species are present in alfalfa plots. On the other hand, in Mexico, Chew (2000) noted there was a high incidence of crown rot in alfalfa plots in the Comarca Lagunera (region of lagoons). In the Mexican state of Guanajuato, F. incarnatum was reported as being the causal agent of alfalfa root rot (Esteban-Santiago et al., 2016). Velásquez-Valle (2001) reported the presence of galls on the roots of alfalfa plants collected in Aguascalientes. Other studies have reported the presence of bulb nematodes (Ditylenchus dipsaci) on alfalfa leaf samples collected in the state of Jalisco (Rosas-Hernández et al., 2017). Alfalfa productivity in this region could increase by implementing phytosanitary measures to reduce the crown rot problem. However, these measures must be solidly based on knowledge of the organisms that could be the potential causes of the disease. The objective of this study was to identify the genera of microorganisms associated with alfalfa plants presenting crown rot symptoms in Aguascalientes, Durango and Zacatecas, Mexico.

Between January and August 2017, samples of alfalfa roots with crown rot symptoms and of the soil surrounding the plants were collected from 30 commercial plots, of which 50, 30 and 20% were in Zacatecas, Aguascalientes and Durango, respectively. Three stunted plants with a few yellow leaves, most of them with wilt symptoms, were randomly collected from each plot. The plants were extracted with as many of the roots as possible, and a subsample of the soil surrounding each plant was taken; this soil was mixed together to obtain a representative compound sample. The crown of each plant was rinsed with tap water and tissue was taken from its center. The tissue was then disinfected and cultivated in Petri dishes containing a potato-dextrose-agar (PDA) culture medium (Velásquez-Valle et al., 2001). The Petri dishes were incubated at 28 ± 1 °C in a bacteriological oven (Felisa, Mod. FE 131) for three or four days. The genera of the resulting colonies of fungi or oomycetes were identified using the taxonomical keys of Barnett (1967) and Watanabe (1994). To extract thread-like nematodes, each soil sample was homogenized and then a 50-g subsample of each was taken and placed in a Baermann funnel, according to the methodology proposed by Cepeda (1995). The genera of the recovered nematodes were identified using the taxonomic keys and information provided by Mai and Mullin (1996), Kanzaki and Giblin-Davis (2012)) and Castillo et al. (2012)). The roots were examined to detect galls caused by the Meloidogyne nematode.

The results showed there were five genera of fungi or oomycetes on the root samples that are potentially phytopathogenic to the crop (Fusarium, Rhizoctonia, Verticillium, Pythium and Phytophthora), while the results of soil samples showed 13 genera of phytoparasite, predator and free-living nematodes (Aphelenchus, Aphelenchoides, Criconemoides, Ditylenchus, Dorylaimus, Helicotylenchus, Mononchus, Pratylenchus, Psilenchus, Rhabditis, Trichodorus, Tylenchus and Xiphinema). The most frequently identified nematodes were Aphelenchus, Rhabditis and Dorylaimus (96.7, 73.3 and 70%, respectively). In contrast, Criconemoides, Trichodorus and Xiphinema were identified the least frequently (3.3% in each case) (Table 1).

Table 1 Frequency of nematode detection in the rhizosphere of alfalfa plants with crown rot symptoms in plots in Aguascalientes, Durango and Zacatecas, Mexico. 

Género Frecuencia de detección (%) Distribución
Aphelenchus 96.7 AGSx, DGOy, ZACz
Aphelenchoides 13.3 DGO, ZAC
Criconemoides 3.3 ZAC
Ditylenchus 56.7 AGS, DGO, ZAC
Dorylaimus 70.0 AGS, DGO, ZAC
Helicotylenchus 6.6 AGS, DGO
Mononchus 16.7 AGS, DGO, ZAC
Pratylenchus 50.0 AGS, DGO, ZAC
Psilenchus 6.6 AGS, ZAC
Rhabditis 73.3 AGS, DGO, ZAC
Trichodorus 3.3 AGS
Tylenchus 33.3 AGS, DGO, ZAC
Xiphinema 3.3 ZAC

x Aguascalientes, y Durango, z Zacatecas.

It should be noted that Ditylenchus was present in approximately 57% of the alfalfa plots sampled in the three states, it is important to highlight a study conducted by Boelter et al. (1985), who indicated the association of D. dipsaci with the death in winter of alfalfa plants and reduced forage yield. Thus, it could be that the damage caused by the nematode in this region of Mexico is underestimated or mistaken for the effect of low winter temperatures. On the other hand, we should highlight that the nematode was not present in samplings conducted by Chew (2000) in alfalfa plots in the Comarca Lagunera. Williams-Woodward and Gray (1999), and Milano de Tomasel and McIntyre (2001) also pointed out that D. dipsaci infestations frequently occur in conjunction with Aphelenchoides ritzemabosi, both in the soil and on alfalfa foliage. In the present study, we detected the simultaneous presence of Aphelenchoides and Ditylenchus in soil samples taken from two alfalfa plots in southeastern Zacatecas. Aphelenchoides was also found in two alfalfa plots in Durango, but not Ditylenchus.

According to Castillo et al. (2012), Pratylenchus penetrans is distributed all over the Americas, where it parasitizes more than 350 hosts, including alfalfa. The results of this study show that specimens of the Pratylenchus genus were found in 50% of the alfalfa plots sampled in the three states. According to Chew (2000), this nematode is present in alfalfa plots in Coahuila and Durango. Mauza and Webster (1982) demonstrated a synergistic interaction between P. penetrans and Fusarium oxysporum populations in alfalfa seedlings. Although F. solani was not pathogenic to alfalfa seedlings, it reduced P. penetrans populations. It is important to point out that in this study the presence of Fusarium in alfalfa plants’ crown tissue coincided with the presence of Pratylenchus in the soil samples, though this information can be confirmed later by extracting nematodes from the crown tissue.

The Aphelenchus genus includes species considered to be biological control agents of fungal microorganisms and facultative phytopathogenic species, although severe damage has not been reported on major agricultural species (Kanzaki and Giblin-Davis, 2012). Aphelenchus avenae is widely distributed on decaying rhizomes, tubers, bulbs and roots. It was found on lesions caused by Pythium arrhenomanes on maize roots (Zea mays) but not on healthy roots (Mai and Mullin, 1996). The high incidence of this nematode (96.7%) found in this study is probably due to the decaying tissues in the plant’s crown.

In one third of soil samples, specimens belonging to Tylenchus were found. According to Thorne (1961), members of the Tylenchus family are among the nematodes most frequently found in the rhizosphere; however, except for T. costatus, they have not been reported as phytoparasites.

A Xiphinema specimen was identified in an alfalfa plot in Zacatecas; it had been previously reported (Chew, 2000) on alfalfa crops in the states of Coahuila and Durango. Wojtowicz et al. (1982) also reported the incidence of this genus on alfalfa in the state of Pennsylvania, USA. Trichodorus was detected in an alfalfa plot in Aguascalientes. It is important to mention that Thorne (1961) had indicated that alfalfa was not a good host of this nematode.

No galls caused by nematodes such as Meloidogyne were detected, and this result is in agreement with that obtained by Chew (2000) in alfalfa plots in the Comarca Lagunera. However, a previous report mentioned the presence of galls caused by this nematode in an alfalfa plot in Aguascalientes (Velásquez-Valle, 2001).

The alfalfa plants collected had scant foliage, most frequently a dull green to yellowish in color, and smaller in size than non-affected plants; other symptoms such as stunting and necrosis were observed as well. The crown of these plants had brown-to-black lesions frequently extending towards the root’s vascular tissues, according to the syndrome described by Uddin and Knous (1991).

Based on these symptoms, five genera of microorganisms were isolated and identified: Fusarium, Rhizoctonia and Verticillium fungi, as well as Phytophthora and Pythium oomycetes (Table 2).

Table 2 Frequency of fungi and oomycete detection on alfalfa plants with crown rot symptoms in plots in Aguascalientes, Durango and Zacatecas, Mexico. 

Frecuencia de aislamiento (%)
Patógeno Aguascalientes Durango Zacatecas Norte centro
Fusarium 100 100 100 100
Rhizoctonia 88.9 83.3 100 93.3
Verticillium 33.3 16.7 66.7 46.7
Phytophthora 0.0 0.0 13.3 6.7
Pythium 83.3 33.3 60.0 53.3

It is worth mentioning the high incidence of Fusarium and Rhizoctonia in alfalfa plots in each state, and consequently, at the regional level; these two fungi are associated with crown rot (Table 2). A group of Fusarium species has been associated with the disease: F. incarnatum, F. solani, F. roseum, F. oxysporum, among others (McKenzie and Davidson, 1975; Mauza and Webster, 1982; Esteban-Santiago et al., 2016). We need to keep in mind that not all the Fusarium species are pathogenic to alfalfa (Mauza and Webster, 1982), so further identification and pathogenicity research needs to be done in order to define the role they play in alfalfa crown rot. According to Chew (2000), Fusarium sp. was the fungus most frequently found on alfalfa plants with crown rot symptoms in the Comarca Lagunera, which is in agreement with the results of this study.

Rhizoctonia solani has been reported to be the causal agent of the damping-off of alfalfa seedlings and of lesions at the base of alfalfa sprouts (Vincelli and Herr, 1992; Fowler et al., 1999). In the Comarca Lagunera, Chew (2000) reported that after Fusarium, Rhizoctonia solani was the fungus found most frequently on alfalfa plants with crown rot.

According to Larkin et al. (1995), some alfalfa root microorganisms, such as Phytophthora and Pythium, have been associated with limited development and establishment of alfalfa populations. Phytophthora was identified in two alfalfa plots in the municipalities of Calera and Luis Moya, Zacatecas. Some Phytophthora species have been classified as pathogenic to alfalfa. For example, alfalfa roots infected by P. megasperma reduced the development rate in adult plants, and severe infections caused symptoms such as yellow foliage, wilting and premature defoliation (Marks and Mitchell, 1971). On the other hand, the incidence of Pythium in the region was 53.3% (Table 2). A similar situation was reported in Sudan (Ao et al., 2018), where the combined incidence of four Pythium species reached 44%.

In conclusion, 13 genera of nematodes were identified (Aphelenchus, Aphelenchoides, Criconemoides, Ditylenchus, Dorylaimus, Helicotylenchus, Mononchus, Pratylenchus, Psilenchus, Rhabditis, Trichodorus, Tylenchus and Xiphinema) in the rhizosphere of alfalfa plants with crown rot symptoms; three genera of fungi (Fusarium, Rhizoctonia, Verticillium) and two of oomycetes (Phytophthora and Pythium) were associated with crown rot on alfalfa plants.

LITERATURA CITADA

Ao S, Bucciarelli B, Dornbusch MR, Miller SS, and Samac DA. 2018. First report of alfalfa (Medicago sativa L.) seed rot, seedling root rot, and damping-off caused by Pythium spp. in Sudanese soil. Plant Disease 102:1043. Disponible en línea: https://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-09-17-1411-PDNLinks ]

Barnett HL. 1967. Illustrated genera of imperfect fungi. Second Edition. Burgess Publishing Company. 225 p. [ Links ]

Boelter HR, Gray AF, and Delaney HR. 1985. Effect of Ditylenchus dipsaci on alfalfa mortality, winterkill, and yield. Journal of Nematology 17:140-144. Disponible en línea: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2618429/ [ Links ]

Castillo P, Stanley J, Inserra RN, and Manzanilla-López RH. 2012. Pratylenchidae-the lesión nematodes. Pp. 411-478. In: Practical Plant Nematology. (Ed. by R.H. Manzanilla-López and N. Marbán-Mendoza). Biblioteca Básica de Agricultura. Guadalajara, Jalisco, México. 883 p. [ Links ]

Cepeda SM. 1995. Prácticas de Nematología Agrícola. Primera edición. Editorial Trillas. 109 p. [ Links ]

Chew MIY. 2000. Enfermedades de la alfalfa. In: Producción y utilización de la alfalfa en la zona norte de México. Libro Técnico No. 2. Campo Experimental La Laguna - INIFAP. 102 p. Disponible en línea: http://biblioteca.inifap.gob.mx:8080/jspui/bitstream/handle/123456789/1938/produccionyutilizaciondelaalfalfaenlazonanortedemexico.pdf?sequence=1Links ]

Esteban-Santiago JM, Leyva-Mir SG, Royan IM, Camacho-Tapia M y Tovar-Pedraza JM. 2016. Identificación del agente causal de la pudrición de la raíz y hongos asociados a semilla de alfalfa (Medicago sativa) en Guanajuato, México. Revista Mexicana de Fitopatología 34 (Suplemento):S59. Disponible en línea: http://rmf.smf.org.mx/suplemento/docs/Volumen342016/Resumen_Poster_S342016.pdfLinks ]

Fowler MC, Miller-Garvin JE, Regulinski DP, and Viands DR. 1999. Association of alfalfa radicle lenght with Rhizoctonia damping off. Crop Science 39:659-661. Disponible en línea: http://agris.fao.org/agris-search/search.do?recordID=US201302918657Links ]

Kanzaki N. and Giblin-Davis RM. 2012. Aphelenchoidea. Pp. 161-208. In: Practical Plant Nematology. (Ed. by R.H. Manzanilla-López and N. Marbán-Mendoza). Biblioteca Básica de Agricultura. Guadalajara, Jalisco, México. 883 p. [ Links ]

Lara MCR y Jurado GP. 2014. Paquete tecnológico para producir alfalfa en el estado de Chihuahua. Sitio Experimental La Campana-INIFAP. Folleto Técnico Núm. 52. Chihuahua, Chih. México. 53 p. Disponible en línea: https://www.producechihuahua.org/paqs/PT-0010Alfalfa.pdfLinks ]

Larkin RP, English JT, and Mihail JD. 1995. Effects of infection by Pythium spp. on root system morphology of alfalfa seedlings. Phytopathology 85:430-435. http://doi.org/10.1094/Phyto-85-430 [ Links ]

Mai WF and Mullin PG. 1996. Plant-parasitic nematodes. Fifth Edition. A pictorial key to genera. Cornell University Press. 277 p. [ Links ]

Marks GC and Mitchell JE. 1971. Penetration and infection of alfalfa roots by Phytophthora megasperma and the pathological anatomy of infected roots. Canadian Journal of Botany 49:63-67. Disponible en línea: https://vdocuments.site/documents/penetration-and-infection-of-alfalfa-roots-by-Phytophthora-megasperma.htmlLinks ]

Mauza BE and Webster JM. 1982. Suppression of alfalfa growth by concommitant populations of Pratylenchus penetrans and two Fusarium species. Journal of Nematology 14:364-367. Disponible en línea: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2618186/Links ]

McKenzie JS and Davidson JGN. 1975. Prevalence of alfalfa crown and root diseases in the Peace river region of Alberta and British Columbia. Canadian Plant Disease Survey 55:121-125. Disponible en línea: https://phytopath.ca/wp-content/uploads/2014/10/cpds-archive/vol55/CPDS_Vol_55_No_4_(121-125)1975.pdfLinks ]

Milano de Tomasel MC and McIntyre GA. 2001. Distribution and biology of Ditylenchus dipsaci and Aphelenchoides ritzemabosi in alfalfa grown in Colorado. Nematropica 31:11-16. Disponible en línea: https://www.researchgate.net/publication/35224467_Distribution_and_biology_of_Ditylenchus_dipsaci_and_Aphelenchoides_ritzemabosi_in_alfalfa_grown_in_ColoradoLinks ]

Rosas-Hernández L, Ramírez-Suarez A, Alcasio-Rangel S, López-Buenfil JA and Medina-Gómez E. 2017. Detection, identification and phylogenetic inference of the stem nematode Ditylenchus dipsaci (Kühn) Filipjev (Nematoda:Anguinidae) affecting alfalfa Medicago sativa L. in Jalisco, México. Revista Mexicana de Fitopatología 35:377-396. DOI: 10.18781/R.MEX.FIT.1703-8 [ Links ]

SIAP. 2018. Sistema de Información Agroalimentaria y Pesquera. https://www.gob.mx/siap (consulta, 22 de Julio de 2018). [ Links ]

Thorne G. 1961. Principles of Nematology. McGraw-Hill Book Company, Inc. 553 p. [ Links ]

Uddin W. and Knous TR. 1991. Fusarium species associated with crown rot of alfalfa in Nevada. Plant Disease 75:51-56. DOI: 10.1094/PD-75-0051 [ Links ]

Velásquez-Valle R. 2001. Geographic and host range of Meloidogyne spp. in north central Mexico. Plant Disease 85:445. https://doi.org/10.1094/PDIS.2001.85.4.445A [ Links ]

Velásquez-Valle R, Medina-Aguilar MM y Luna-Ruiz JJ. 2001. Sintomatología y géneros de microorganismos asociados con las pudriciones de la raíz de chile (Capsicum annuum L.) en el norte centro de México. Revista Mexicana de Fitopatología 19:175-181. Disponible en línea: http://www.redalyc.org/pdf/612/61219207.pdfLinks ]

Vincelli PC and Herr LJ. 1992. Two diseases of alfalfa caused by Rhizoctonia solani AG-1 and AG-4. Plant Disease 76:1283. DOI: 10.1094/PD-76-1283B [ Links ]

Watanabe T. 1994. Pictorial atlas of soil and seed fungi. Morphologies of cultured fungi and key to species. CRC Press, Inc. 411 p. [ Links ]

Williams-Woodward JL and Gray FA. 1999. Seasonal fluctuations of soil and tissue populations of Ditylenchus dipsaci and Aphelenchoides ritzemabosi in alfalfa. Journal of Nematology 31:27-36. Disponible en línea: http://journals.fcla.edu/jon/article/view/67032Links ]

Wojtowicz MR, Golden AM, Forer LB, and Stouffer RF. 1982. Morphological comparisons between Xiphinema rivesi Dalmasso and X. americanum Cobb populations from the Eastern United States. Journal of Nematology 14:511-516. Disponible en línea: http://journals.fcla.edu/jon/article/view/65411/63079Links ]

Received: June 13, 2018; Accepted: August 03, 2018

Creative Commons License Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons