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Revista de investigación clínica

versión impresa ISSN 0034-8376

Rev. invest. clín. vol.59 no.2 México mar./abr. 2007

 

Artículo original

 

Helicobacter pylori infection and thrombocytopenia: A single-institution experience in Mexico

 

Infección por Helicobacter pylori y trombocitopenia: Experiencia en una sola institución mexicana

 

Roberto A. Estrada-Gómez,* Israel Parra-Ortega,* Carlos Martínez-Barreda,* Guillermo J. Ruiz-Argüelles*,**

 

* Laboratorios Clínicos de Puebla.

** Centro de Hematología y Medicina Interna de Puebla.

 

Correspondence and reprint request:
Guillermo J. Ruiz-Argüelles, M.D.
Centro de Hematología y Medicina Interna
8B Sur 3710, Col. Anzures
72530, Puebla, Pue.

Correo electrónico: gruizl@clinicaruiz.com

 

Recibido el 24 de agosto de 2006.
Aceptado el 31 de enero de 2007.

 

ABSTRACT

The association between gastrointestinal H. pylori infection and thrombocytopenia was studied in a single institution in Mexico, over a 5-year period. In 99 individuals with H. pylori infection, the prevalence of thrombocytopenia was 14%, whereas in 23 consecutive patients with chronic refractory thrombocytopenic purpura, the prevalence of H. pylori infection was 60%, this figure being similar to that informed in the general population of Mexico (66%); the association between thrombocytopenia and H. pylori infection was not significant. In 14 patients who were found to have both thrombocytopenia and H. pylori infection, eradication treatment was given and the platelet count recovered in three. It is not still clear if detection of H. pylori infection should be routinely included in the initial workup of chronic thrombocytopenia.

Key words. H. pylori. Thrombocytopenia. Purpura. Mexico.

 

RESUMEN

Se investigó la asociación entre infección del tubo digestivo por H. pylori y trombocitopenia en una sola institución en México, en un periodo de cinco años. En 99 individuos infectados por H. pylori, la prevalencia de trombocitopenia fue de 14%; por otro lado, en 23 pacientes consecutivos con púrpura trombocitopénica crónica refractaria, la prevalencia de infección por H. pylori fue de 60%, cifra similar a la descrita para la población general de nuestro país, de alrededor de 66%; en consecuencia, la asociación entre trombocitopenia e infección por H. pylori no fue significativa. En 14 pacientes en quienes coexistieron púrpura trombocitopénica e infección por H. pylori, se administró tratamiento de erradicación de la bacteria y la cuenta de plaquetas se normalizó en tres. Los datos apoyan otras publicaciones que muestran falta de asociación entre estas variables y son insuficientes para recomendar si es prudente o no investigar la infección por H. pylori en el estudio inicial de todos los pacientes con púrpura trombocitopénica.

Palabras clave. H. pylori. Trombocitopenia. Púrpura. México.

 

INTRODUCTION

Helicobacter pylori (H. pylori) is a gram-positive, spiral-shaped bacterium which resides in the stomach mucosa. Since its discovery by Marshall and Warren,1 H. pylori has been considered as the etiologic agent of gastritis and peptic ulcer, and has been found to be associated with gastric cancer, mucosa-associated lymphoid tissue lymphoma,2-4 etc. More recently, H pylori has been found to be associated with ischemic heart disease5 and a number of autoimmune disorders, such as rheumatoid arthritis,6 autoimmune thyroiditis,7 Sjögren's syndrome,8 Henoch-Schönlein purpura,9 autoimmune thrombocytopenic purpura (AITP)10,11 and others.

AITP is an acquired bleeding disorder in which autoantibodies bind to the platelet surface, leading to platelet destruction.12'13 The triggering mechanisms of the production of platelet autoantibodies are still poorly understood.14 There is information suggesting that the immune process leading into the platelet destruction may be associated with H. pylori infection10-11 and that eradication of the bacteria may resolve the thrombocytopenia.15 We analyze herein data concerning the association between H. pylori infection and thrombocytopenia, in a single institution in Mexico.

 

MATERIAL AND METHODS

Laboratorios Clínicos de Puebla (LCP) and Centro de Hematología y Medicina Interna de Puebla (CHMI) are parts of the Clínica Ruiz de Puebla, located in Puebla, México. All patients refered to LCP between July 2002 and June 2006 to investigate the presence of H. pylori in stools were included in this study; a subset of these individuals in whom the platelet count was also assessed was analyzed separately. On the other hand all patients studied prospectively in the CHMI after July 2002 to define the etiology of chronic (more than 6 months) thrombocytopenia (less than 100 x 109/L platelets), who had failed to respond to at least a single course of corticosteroids were also prospectively included in the study; the investigation of H. pylori was done in all of them as part of the initial laboratory work-up. Patients with overt autoimmune disease, malignancy or liver disease were excluded. The platelet counts were assessed by means of flow cytometry (Beckman Coulter HmX, Miami, FL), whereas H. pylori infection was assessed by means of an enzyme immunoassay for the detection of H. pylori antigens in stool (Premiere Platinum HpSA® Plus, Meridian Bioscience, Cincinnati, OH). No other methods were used to define the presence of H. pylori. The association of H. pylori infection and thrombocytopenia was analyzed en two ways:

1. In patients with chronic thrombocytopenia, the prevalence of H. pylori infection was studied.

2. In patients with H. pylori infection, the prevalence of thrombocytopenia was assessed.

 

RESULTS

742 individuals in a 5-year period were refered to Laboratorios Clínicos de Puebla to asses the digestive tract infection by H. pylori; in a subset of 237 of these patients the platelet count was also measured. In this group of 237 patients, H. pylori infection was identified in 99 patients (42%); the prevalence of thrombocytopenia in these 99 patients was 14% (14/99). On the other hand, nine patients with a low platelet count were identified in the 138 individuals without H. pylori infection (6.5%). The association between thrombocytopenia and H. pylori infection was not significant using the chi square test, see table 1.

On the other hand, in 23 consecutive patients with chronic refractory thrombocytopenic purpura prospectively studied and treated at the CHMI H. pylori infection was done as part of the initial laboratory workup; in all these individuals overt autoimmune diseases had been ruled out. In this group the prevalence of H. pylori infection was 60% (14/ 23); this figure being similar to that informed in the general population of Mexico, which is around 66%.16 In the 14 patients who were found to have both thrombocytopenia and H. pylori infection, quadruple-drug (amoxicillin, clarithromycin, bismuth subsalicylate and pantoprazole) treatment was given during a two-week period, the platelet count recovering in three of them; the table 2 shows some features of the three individuals who normalized their platelet counts; H. pylori was cleared in all the patients, Patients numbers 2 and 3 have had sustained responses for over 150 and 210 days respectively, whereas patient number 1 had a transient response for 60 days with a subsequent relapse of the thrombocytopenia which lead into splenectomy. In this group of patients, median age was 53 years (range 7 to 78), there were 8 males and 6 females, whereas the platelet count has a median of 74 x 109/L (range 5 to 98).

 

DISCUSSION

Several lines of direct and indirect evidence suggest that infectious agents may influence the occurrence or the course of some autoimmune diseases.17 The involvement of H. pylori has been suggested in various autoimmune diseases.6-9 The role of H. pylori infection in the pathogenesis of AITP has been suggested and significant increases in the platelet count have been were reported after eradication of H pylori infection;10-12 however, its role in the pathogenesis of AITP is still controversial.

Emilia, et al.10 and Gasbarrini, et al.15 both in Italy, reported that 43% and 61% respectively of a total of 48 AITP cases were infected with Hpylori, whereas the prevalence of H. pylori infection in the general population in Italy is 45%.19 On the other hand Kohda, et al,18 in Japan, found that H. pylori was present in 62.5% of 40 AITP patients, the prevalence of the infection in the general population in Japan being 25-45%.20 In the study which we are informing, we found a prevalence of H. pylori infection of 60% in AITP, whereas that in the general population in Mexico is 66%.16 All these figures indicate that the prevalence of H. pylori infection in patients with AITP is not very different from that informed in the general population in several countries, this being one possible reason of the lack of association between these two variables.

On the other hand, eradication of H. pylori infection has been informed to result in resolution of the thrombocytopenia in some, but not all cases.15,21,22 We delivered quadruple treatment to all the 14 patients with chronic thrombocytopenia who were found to be infected by H. pylori, and in three cases (21%) an increase in the platelet count was observed; these data are comparable to those informed by Jarque, et al., in Spain, who recorded platelet increases in 3 of 56 patients (5%) given eradication treatment.22

In conclusion, this study conducted in a single institution in Mexico fails to show a significant association between H. pylori infection and thrombocytopenia, despite the low number on individuals studied. However, since some cases were observed to increase the platelet counts after eradication of the bacteria, it may be adequate to deliver this simple and harmless treatment to patients with chronic refractory thrombocytopenia, prior to engaging in more aggressive or complicated medical actions. It is not still clear if detection of H. pylori infection should be routinely included in the initial workup of AITP.

 

REFERENCES

1. Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 1984; i: 1311-14.        [ Links ]

2. Suerbaum S, Michetti P. Helicobacter pylori infection. N Engl J Med 2002; 347: 1175-86.        [ Links ]

3. Ando K, Shimamoto T, Tauchi T, Ito Y, Kuriyama Y, Gotoh A, Miyazawa K, et al. Eradication therapy for Helicobacter pylori really improve the thrombocytopenia in idiopathic thrombocytopenic purpura? Our experience and a literature review. Int J Hematol 2003; 77: 239-44.        [ Links ]

4. Veneri D, Franchini M, Gottardi M, D'Adda M, Ambrosetti A, Krampera M, et al. Efficacy of Helicobacter pylori eradication in raising platelet count in adult patients with idiopathic thrombocytopenic purpura. Haematologia 2002; 87:1177-9.        [ Links ]

5. Murray LJ, Bamford KB, O'Reilly DPJ, McCrum EE, Evans AE. Helicobacter pylori infection: relation with cardiovascular risk factors, ischaemic heart disease, and social class. Br Heart J 1995; 74: 497-501.        [ Links ]

6. Zentilin P, Savarino V, Garnero A, Accardo S, Seriolo B. Is Helicobacter pylori infection a risk factor for disease severity in rheumatoid arthritis? Gastroenterology 1999; 116: 503-04.        [ Links ]

7. De Luis DA, Várela C, de la Calle H. Helicobacter pylori infection is markedly increased in patients with autoimmune atropic thyroiditis. J Clin Gastroenterol 1998; 26: 259-63.        [ Links ]

8. Figura N, Giordano N, Burroni D, Macchia G, Vindigni C, Gennari C, Bayeli PF. Sjogren's syndrome and Helicobacter pylori infection. European J Gastroenterol Hepatol 1994; 6: 321-2        [ Links ]

9. Reinauer S, Megahed M, Goertz G, Ruzicka T, Borchard F, Su-santo F, Reinauer H. Schonlein-Henoch purpura associated with gastric Helicobacter pylori infection. J Am Acad Dermatol 1995; 33: 876-9.        [ Links ]

10. Emilia G, Longo G, Luppi M, Gandini G, Morselli M, Ferrara L, et al. Helicobacter pylori eradication can induce platelet recovery in idiopathic thrombocytopenic purpura. Blood 2001; 97: 812-14        [ Links ]

11. Tohda S, Ohkusa T. Resolution of refractory idiopathic thrombocytopenic purpura after eradication of Helicobacter pylori. Am J Hematol 2000; 65: 329-30.        [ Links ]

12. Grimaz S, Damiani D, Brosolo P, Skert C, Geromin A, de Pretis G. Resolution of thrombocytopenia after treatment for Helicobacter pylori: a case report. Haematologica 1999; 84: 283-84.        [ Links ]

13. George JN, el-Harake MA, Raskob GE. Chronic idiopathic thrombocytopenic purpura. N Engl J Med 1994; 331: 1207-11.        [ Links ]

14. Michel M, Khellaf M, Desforges L, Lee K, Schaeffer A, Godeau B, Bierling P. Autoimmune thrombocytopenic purpura and Helicobacter pylori infection. Arch Intern Med 2002; 162: 1033-6.        [ Links ]

15. Gasbarrini A, Franceschi F, Tartaglione R, Landolfi R, Pola P, Gasbarrini G. Regression of autoimmune thrombocytopenia after eradication oí Helicobacter pylori. Lancet 1998; 352: 878.        [ Links ]

16. Torres J, Leal-Herrera Y, Pérez-Pérez G, Gómez A, Camorlinga-Ponce M, Cedillo-Rivera R, et al. A community based seroepidemiologic study of Helicobacter pylori infection in Mexico. J. infect. Dis 1998, 178: 1089.        [ Links ]

17. Benoist C, Mathis D. Autoimmunity: the pathogen connection. Nature 1998; 394: 227-8.        [ Links ]

18. Kohda K, Kuga T, Kogawa K, Kanisawa Y, Koike K, Kuroiwa G, et al. Effect of Helicobacter pylori eradication on platelet recovery in Japanese patients with chronic idiopathic thrombocytopenic purpura and secondary autoimmune thrombocytopenic purpura. Br J Hematol 2002; 118: 584-8.        [ Links ]

19. Palli D, Decarli A, Cipriani F, Sitas F, Forman D, Amadori D, et al. Helicobacter pylori antibodies in areas of Italy at varying gastric cancer risk. Cancer Epidemiol Biomarkers Prev 1993; 2: 37-40.        [ Links ]

20. Asaka M, Kimura T, Kudo M, Tadeka H, Miyazaki T, Miki K, Garaham DY. Relationship of Helicobacter pylori to serum pepsinogens in an asymptomatic Japanese population. Gastroenterology 1992; 102: 760-6.        [ Links ]

21. Banford KB, Andersen L. Host response. Curr Opin Gastroenterol 1997; 13: 25-30.        [ Links ]

22. Claeys D, Faller G, Appelmelk BJ, et al. The gastric H+/K+ -ATPase is a major autoantigen in chronic Helicobacter pylori gastritis with body mucosa atrophy. Gastroenterology 1998; 115: 340-7.        [ Links ]

23. Jarque I, Andrew R, Llopis I, et al. Absence of platelet response after eradication of Helicobacter pylori infection in patients with chronic idiopathic thrombocytopenic purpura. Br J Hematol 2001; 115: 1002-3.        [ Links ]

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