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Scientia fungorum

versión On-line ISSN 2594-1321

Resumen

HUARHUA, Medali et al. First Report of Fusarium oxysporum f. sp. lycopersici race 1 isolated of Tomato (Solanum lycopersicum L.) comes from the Central Coast of Peru. Sci. fungorum [online]. 2020, vol.50, e1257.  Epub 10-Mar-2021. ISSN 2594-1321.  https://doi.org/10.33885/sf.2020.50.1257.

Background:

Fusarium oxysporum f. sp. lycopersici (Fol) causes wilt symptoms on tomato. Fusarium species can be identified by morphological characteristics. However, in order to detect special form, races or pathogenic forms, molecular methods and differential cultivars are used. Currently, in Peru there is no report of the identification of races of Fusarium oxysporum f. sp. lycopersici.

Objectives:

Identify by molecular techniques and the use of differential cultivars, Fusarium oxysporum races associated with tomato wilt from the coast of Peru.

Methods:

Morphological Identification of eight Fusarium isolates from tomato were done followed by phylogenetic analysis based on the intergenic spaces (IGS) of DNA r. In addition, avirulence genes (SIX4, SIX3 and SIX1) and fragments of the endo (pg1) and exo (pgx4) polygalacturonase genes of the pathogen were detected. Pathogenicity tests were carried out on tomato differential cultivars (Ponderosa, Momotaro, Momotaro 8 and Block).

Results and conclusions:

According to the molecular techniques and pathogenicity test, the HEI 11 isolate was identified as Fol race 1.

Palabras llave : Cultivars; IGS; genes SIX; polygalacturonase; xylem.

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