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Veterinaria México OA

versão On-line ISSN 2448-6760

Resumo

ANTONIO-MARCOS, E. et al. Can C-phycocyanin be used as a sperm protector?. Veterinaria México OA [online]. 2018, vol.5, n.4. ISSN 2448-6760.  http://dx.doi.org/10.22201/fmvz.24486760e.2018.4.642.

C-phycocyanin (C-PC) is a biocompound extracted from Arthrospira maxima. It is a chromophore-protein complex, with antioxidant properties. Its ability to prevent oxidative stress allows for diverse medical applications. This study evaluates the use of C-PC as a protein supplement and an antioxidant for in-vitro sperm preservation in a short-term extender. Viability, progressive motility, DNA damage and percentage of reactive oxygen species where assessed in Swine (Sus scrofa domesticus) sperm stored for up to 72 hours at 4 °C. Treatments with C-PC had the following concentrations: 0, 34.5, 69, 138 and 207 µg mL−1. Progressive motility and percentage of sperm with undamaged DNA were unchanged (20%) after storage for 48 hours using the 138 ug mL-1 C-PC concentration.

Palavras-chave : C-phycocyanin; sperm refrigeration; DNA damage; progressive motility; extender.

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