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Veterinaria México OA

versão On-line ISSN 2448-6760

Resumo

MARTINEZ-IBARRA, José Luis et al. Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos. Veterinaria México OA [online]. 2018, vol.5, n.4. ISSN 2448-6760.  http://dx.doi.org/10.22201/fmvz.24486760e.2018.4.491.

The effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5 µM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5 µM (81.3 %) and 2 µM (72 %) resveratrol compared to that of the control group (0 µM) (74.2 %), but the rate significantly decreased at 5 µM (56 %) (p < 0.05). When the oocyte ROS levels were determined, no significant differences among the groups were observed (p > 0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5 µM resveratrol showed higher (p < 0.05) compacted morula rates (10.7 %) compared to the embryos obtained from the oocytes treated with 0, 2 and 5 µM (6.2, 0 and 0 %, respectively). Regarding embryo morphological quality, the embryos from the oocytes treated with 0.5 µM resveratrol showed a lower rate of poor quality morulae (4.7 %) in comparison to those treated with 0, 2 and 5 µM (23.8, 23.3 and 33.3 %, respectively) (p < 0.05). In conclusion, resveratrol showed no significant improvement on the IVM or ROS levels in domestic ovine oocytes. However, treatment with 0.5 µM resveratrol during IVM improved embryo quality and promoted morulae compaction of Ovis aries handmade cloned embryos.

Palavras-chave : Resveratrol; Ovis aries; In vitro maturation; Reactive oxygen species; Embryo development; Handmade cloning.

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