Resumo

JUAREZ-MORENO, Karla; ANGUIS DELGADO, Kathya; PALESTINA ROMERO, Brenda  e  VAZQUEZ-DUHALT, Rafael. Evaluating the toxicity of nanomaterials in three-dimensional cellular models. Mundo nano [online]. 2020, vol.13, n.25, pp.157-171.  Epub 25-Nov-2020. ISSN 2448-5691.  https://doi.org/10.22201/ceiich.24485691e.2020.25.69608.

Traditionally, in vitro evaluations to determine the cytotoxic effect of nanomaterials in cell cultures have been carried out in two-dimensional cultures, because the protocols to assess it have been adapted from those used in toxicology. However, the interactions between cells are much more complex than those found in a monolayer arrangement. Thus, this is the main reason to promote the implementation of three-dimensional cell cultures also known as spheroids, to assess the effect of nanomaterials on cell cultures. Abundant information supports the idea that spheroids represent a better model for the study of cellular responses, since they emulate more precisely the cellular junctions, communication and physiology that occur in a tissue within an in vivo model. Herein, we discuss some points about the development of 3D cultures as a new and better methodology for nanotoxicological evaluations.

Palavras-chave : spheroids; nanotoxicology; cytotoxicity; cell culture.

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