Abstract

QUINONES-AGUILAR, Evangelina Esmeralda; REYES-TENA, Alfredo; HERNANDEZ-MONTIEL, Luis Guillermo  and  RINCON-ENRIQUEZ, Gabriel. Bacteriophages in the biological control of Pseudomonas syringae pv. phaseolicola, causal agent of halo blight in bean. Ecosistemas y recur. agropecuarios [online]. 2018, vol.5, n.14, pp.191-202. ISSN 2007-901X.  https://doi.org/10.19136/era.a5n14.1159.

Pseudomonas syringae pv. phaseolicola (Psph) is the causal agent of halo blight in bean. Management of this disease is based on agricultural antibiotics or copper-based compounds. An alternative is biological control with bacterial viruses (bacteriophages). Therefore, the aim of this study was to evaluate the effect of bacteriophages native to the state of Zacatecas in the control of virulent strains of Psph. Three experiments were carried out: 1) the pathogenicity and virulence of six strains of Psph and the reference strain 1448A were evaluated in green beans and Negro Bolita variety bean plants; 2) the virulence of the three most aggressive strains was evaluated in the Flor de Mayo variety, which shows greater tolerance to halo blight; and 3) seven bacteriophages were evaluated to determine the biological control of the blight in the native strain Psph1 and strain 1448A in Flor de Mayo variety green beans. The halo blight area was quantified in mm2; it was found that the six native Psph strains were pathogenic in green beans and bean plants. Virulence from highest to lowest degree was: 1448A (7.5 mm2); Psph67 (5.3 mm2) and Psph1 (3.8 mm2). Only one bacteriophage (F2) significantly reduced (p ≤ 0.05), by 60%, the area caused by strain 1448A in green beans. The results suggest that bacteriophage technology could be a strategy for the biological control of halo blight in bean in Mexican agriculture.

Keywords : Biocontrol; Phaseolus vulgaris; bacteriophages; halo blight; bacterial lysis.

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