Abstract

REYES-SANDOVAL, Raúl Miguel et al. Detection of Neospora Caninum by PCR nested in leukocytes from dairy bovines. Ecosistemas y recur. agropecuarios [online]. 2017, vol.4, n.12, pp.563-570. ISSN 2007-901X.  https://doi.org/10.19136/era.a4n12.1288.

Seropositivity to Neospora caninum was evaluated by Enzyme-Linked ImmunoSorbent Assay (ELISA) and the DNA was detected by PCR nested with external initiators Np 21-4 and internal Np 9-10 in cow leukocytes from the small-scale milk production system in Amecameca, State of México. A herd with 34 adult females was studied, performing follow-up with blood sampling for five months. Also, two brain samples from aborted fetuses were analyzed and the sequence of the amplicons in the Genbank was verified. Consistency between techniques was compared using the Kappa test. ELISA seroprevalence was 85.3 %; and DNA of the parasite was detected in 89.4 % of the cows. A brain sample was positive. The similarity of the amplicons ranged from 91 to 96 %, the Kappa Índex was 0.41. The use of leukocytes increases the likelihood of amplifying N. caninum DNA.

Keywords : Detection; leukocytes; Neospora caninum; PCR.

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