Abstract

MONROY-TORRES, Rebeca; LINARES-SEGOVIA, Benigno  and  RAMIREZ-GOMEZ, Xóchitl Sofía. Development of an in vitro antibiotics detection technique in samples of beef liver, pork and chicken. CienciaUAT [online]. 2015, vol.9, n.2, pp.68-73. ISSN 2007-7858.

The discovery of antibiotics has enabled scientist to save millions of lives. However, their effectiveness has been declining at the same time that microorganisms are developing resistance due to the continuous application of antibiotics during the rearing of farmed animals' production. It is therefore necessary to develop techniques for the early detection of antibiotics presence in food. A cross-sectional and preliminary study was conducted to establish the feasibility to determine the presence of antibiotics in samples of beef, pork and chicken livers by inhibiting the Escherichia coli ATCC25922 strain growth employing the Kirby-Bauer method. Fifteen Petri plates (five foreach liver sample: beef, pork and poultry) containing the Mueller Hinton agar were inoculated with an antibiotic sensitive E. coli ATCC25922 strain. In each agar plate a portion of liver of 5 mm of diameter was placed 1 cm away from each inoculum. The plates were incubated at 35 OC for 24h and the presence of inhibition halos were evaluated. It was found that only one of the fiftheen liver samples analyzed, corresponding to a beef liver, showed an inhibition halo higher than 30 mm. The obtained results indicate that the use of this technique adequate to detect the presence of antibiotics in meat products.

Keywords : Antibiotics; Escherichia coli; liver; inhibitory effect.

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