Abstract

AVILA-TREVINO, Jazmín A. et al. Morphogenic responses in the in vitro propagation of pecan (Carya illinoinensis [Wangenh] K. Koch). Rev. Chapingo ser. cienc. for. ambient [online]. 2013, vol.19, n.3, pp.469-481. ISSN 2007-4018.  https://doi.org/10.5154/r.rchscfa.2013.09.037.

Embryogenic and organogenic responses in pecan (Carya illinoinensis [Wangenh] K. Koch) were observed as a result of the in vitro cultivation of segments of leaves, axillary buds and zygotic embryos. Necrosis was controlled through the use of activated carbon (AC: 1%), polyvinylpyrrolidone (0.1 %), silver nitrate (AgNO₃: 1 %), citric acid (150 mg·L^-1) and ascorbic acid (100 mg·L^-1, in both light and darkness. Murashige and Skoog base medium (MS) was used, supplemented with 0.40 mg·L^-1 of thiamine, 100 mg·L^-1 of myo-inositol, 3 % saccharose, incorporating 2,4-D for leaves, thidiazuron (TDZ) for embryos, and combinations of benzyladenine (BA), kinetin (KIN) naphthalenacetic acid (ANA) and indolebutyric acid (AIB) for axillary buds. Tissue necrosis was reduced by 75 % and 83 % adding CA and AgNO₃, respectively. 33 % and 66 % of embryogenic callus originated from leaves, using 1 and 3 mg·L^-1 of 2,4-D. The highest callus production (58 %) from embryos was obtained from the concentration of 3 mg·L^-1 of TDZ. In axillary buds, the combination of KIN (3.0 μM), BA (1.0 μM) and AIB (0.3 μM) increased the number of leaves and seedlings, as well as shoot length.

Keywords : Explants; callogenesis; antioxidants; growth regulators.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )