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Revista Chapingo serie ciencias forestales y del ambiente

versão On-line ISSN 2007-4018versão impressa ISSN 2007-3828


VIDAL COB-UICAB, José et al. Potential of organogenesis as a strategy for in vitro propagation of Fitzroya cupressoides in Austral South America. Rev. Chapingo ser. cienc. for. ambient [online]. 2011, vol.17, n.3, pp.423-433. ISSN 2007-4018.

Fitzroya cupressoides, commonly known as Alerce in South American Spanish, is a conifer species native to southern South America, where its wood is highly prized for its beauty and durability. This study evaluated the effect of hormonal components supplemented with culture media on the organogenic process of Fitzroya cupressoides and analyzed the in vitro regeneration process by histological studies. The Quorin-Lepoivre (QL) basic culture medium, supplemented with different concentrations of indole 3-butyric acid (IBA), 6-benzylaminopurine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D), was tested using nodal segments and acicular leaves. A completely randomized design with five balanced replications was used; the experimental unit was a glass jar containing an explant, while a petri dish containing four foliar explants was used for inducing callogenesis. The treatment factor had significant (P = 0.001) effects on the response variables number of shoots and caulinary elongation. Duncan's multiple range test confirmed that the treatments that had significant differences were 0.1 mg·L-1 of IBA and 1.5 mg·L-1 of BA for number of shoots per explant and 0.1 mg·L-1 IBA and 1.0 mg·L-1 of BA for caulinary elongation. Histological studies demonstrated the proliferation of meristematic structures from sub-epidermal tissue.

Palavras-chave : 2,4-dichlorophenoxyacetic acid (2,4-D); callogenesis; nodal segments; in vitro propagation.

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