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Revista bio ciencias
versión On-line ISSN 2007-3380
Resumen
SOTO BELTRAN, M.; QUINONES, B.; IBARRA RODRIGUEZ, A. y AMEZQUITA-LOPEZ, B. A.. Use of Membrane Filtration for the Recovery of Campylobacter from Raw Chicken Carcasses Purchased at Retail Markets in Culiacan, Sinaloa, Mexico. Revista bio ciencias [online]. 2020, vol.7, e698. Epub 24-Jul-2020. ISSN 2007-3380. https://doi.org/10.15741/revbio.07.e698.
Campylobacter jejuni and Campylobacter coli are responsible for human gastroenteritis worldwide, and the consumption of raw and undercooked poultry contributes to human infections. The present study examined the presence of C. jejuni and C. coli from retail chicken carcasses, purchased from local markets in the Culiacan Municipality in Sinaloa, Mexico. To improve the recovery of Campylobacter, the package liquid from the raw chicken carcasses was subjected to membrane filtration after an overnight enrichment under microaerophilic conditions. The presence of C. jejuni and C. coli was further determined by PCR amplification of the lpxA gene. Presumptive Campylobacter colonies were recovered from 43 % (13/30) of the chicken carcasses purchased from 64 % (7/11) of the retail markets examined. Genotyping assays detected C. jejuni in 33.3 % (10/30) of the carcasses examined. This study identified for the first time C. jejuni in raw chicken carcasses in Northwestern Mexico and provided valuable information for monitoring Campylobacter in retail meats.
Palabras llave : Campylobacter jejuni; Campylobacter coli; poultry; foodborne pathogen; food safety.