Abstract

LEMUS-AVALOS, G. et al. Effect of diets with avocado meal on lipids in muscle, antioxidants and gene expression in finished pigs. Revista bio ciencias [online]. 2020, vol.7, e968.  Epub Apr 28, 2021. ISSN 2007-3380.  https://doi.org/10.15741/revbio.07.e968.

The aim of this investigation was to assess the effect level of inclusion of avocado meal (AM) at 0, 5 and 10% in pigs diet for 56 days before their slaughter, on effects in regard to proximal chemical composition, presence of antioxidants, lipid modulation in the Longissimus dorsi and gene expression in the Longissimus dorsi muscle and liver tissues. Eight male pigs of 55 ± 3 kg of initial weight were used in each diet group to compare them under a completely random design, with variance analysis and Tukey test. The gene expression in each tissue was analyzed with a mixedeffects model, comparing the values of change or fold change (FC) of diets with AM against diet control. Levels of γ-tocopherol, total phenolic compounds, antioxidant activity ABTS and DPPH are increased linearly with the inclusion of AM in the diet, decreasing intramuscular fat. In AM5 against AM0, the high expression of lipogenic genes (ACACA, ACP and FASN) in the Longissimus dorsi muscle was associated with the increase of arachidonic fatty acids (20:4-n6) and eicosapentaenoic (20:3-n3). Fatty acid esterification (SCD) was associated with decreased stearic acid (18:0) and increased higher 18:1/18:0 ratio. With AM10 against AM0, the expression of ACACA, SCD, ACP, and FASN were low in both tissues, decreasingin the muscle Longissimus dorsi palmitic acid (16:0); increased: arachidonic (20:0) and linoleic (18:2 n6) acids, PUFA, PUFA/SFA and PUFA/MUFA.

Keywords : Avocado meal; antioxidants; muscle fatty acids; gene expression; pigs; swine diet.

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