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Revista mexicana de ciencias forestales

versión impresa ISSN 2007-1132


GIJON-HERNANDEZ, Adriana Rosalía et al. Identification of the causal agent of antracnosis in the cultivation of the rubber tree [Hevea brasiliensis (Willd. ex A. Juss.) Müll. Arg.]. Rev. mex. de cienc. forestales [online]. 2019, vol.10, n.56, pp.136-148.  Epub 30-Abr-2020. ISSN 2007-1132.

In 2016, a disease developed in clonal rubber tree gardens (clone IAN-710) in San Juan Bautista Tuxtepec, Oaxaca, the symptoms were observed in new leaves, buds and stems characterized by small watery spots that become in circular to irregular necrotic lesions, dark cream to black with yellow edges. Symptomatic samples were taken and 17 colonies were isolated in PDA media, seven from the stem and 10 from the leaves. The pathogen was determined using traditional techniques, PCR-Sequencing with the pair of ITS5 and ITS4 primers that amplified a 550 bp fragment. The phylogenetic analysis was performed with Bayesian inference with 1 000 000 generations and a final standard deviation of 0.008. The isolates presented morphological characteristics of the Colletotrichum genus, while phylogenetic analyzes indicated that the isolates were grouped within the species of the Colletotrichum gloeosporioides complex.

Palabras llave : Antracnose; phylogenetic; phytopathogen; fungus; rubber; PCR.

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