Resumo

CASTILLO-MARTINEZ, Carlos R. et al. Regeneration of Paulownia elongata Steud: Plants by direct organogenesis. Rev. mex. de cienc. forestales [online]. 2012, vol.3, n.10, pp.41-49. ISSN 2007-1132.

Paulownia elongata is an oriental fast-growing forest species that was introduced in Mexico at the end of 1998, as an option for wood commercial plantations, since its timber is light, which favours its use in furniture manufacturing, handicrafts, music instruments and in interior finish. The aim of this work was to determine the best in vitro culture conditions for plant regeneration of Paulownia elongata through several types of explants from steam segments (internode), leaves and petiole, which resulted in a protocol for direct organogenesis that can be used in genetic transformation systems. In all cases, to the basal MS medium were added 6-benzyladenine (BA) from 5 mg L^-1 to 1.0 mg L^-1 and naphtalenacetic acid (NAA) at different concentrations. Response was obtained in all of the explants with the 5 mg L^-1 BA and 1.0 mg L^-1 NAA combination; however internodal segments showed a better bud induction, by producing 83% explants with shoots and 1.52 shoots per explant with the 4 mg L^-1 BA and 0.2 mg L^-1 NAA combination, in contrast to those of petiole segments that produced their best results as 53% explants with shoots and 0.62 shoots per explant, with a 7 mg ^-1 BA and 0.2 mg L^-1 NAA mix.

Palavras-chave : 6-Benciladenine; naphtalenacetic acid; explants; micropropagation; Paulownia elongate Steud.; forest plantations.

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