Abstract

MEDINA ESPARZA, Leticia Esperanza; LUNA OSEGUERA, Ricardo de; VITELA MENDOZA, Irene Victoria  and  CRUZ VAZQUEZ, Carlos. Detection of Neospora caninum from slaughtered dairy cattle in Aguascalientes, Mexico. Rev. mex. de cienc. pecuarias [online]. 2018, vol.9, n.3, pp.408-419. ISSN 2448-6698.  https://doi.org/10.22319/rmcp.v9i3.4538.

The objective was to detect the presence of Neospora caninum in tissues, serum and peripheral blood of slaughtered dairy cattle in abattoirs of Aguascalientes, Mexico. Samples of spinal cord, heart, liver, blood and blood serum were collected from 71 adult animals and were processed as follow. Serum samples were processed by ELISA to detect anti-N. caninum antibodies, while the others were subjected to the nested PCR test to determine the frequency of parasite DNA in different tissues as well as blood. Overall frequency of seropositive animals was 41 % (29/71, 95% CI 29-53) with a range of 33 to 57 %. The frequency of detection of N. caninum DNA in the blood was 44 % (31/71, 95% CI 32-55), with a range between 38 and 50 %; in spinal cord samples of 32 % (27/71, 95% CI 27-50), with a range of 2 to 38 %; in heart samples, 22 % (16/71, 95% CI 13-34), with a range of 4 to 33 %, and in the liver samples, 32 % (23/71, 95% CI 22- 44), with a range of 28 to 57 %. The concordance between the ELISA test and PCR in blood was k= 0.36, of ELISA and PCR in tissues k= 0.07 and of PCR in blood and PCR in tissues k = 0.61. Only eleven cows were positive for all three tests.

Keywords : Neospora caninum; Dairy cattle; DNA; PCR; Blood; Tissues.

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