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Revista mexicana de ciencias pecuarias

versión On-line ISSN 2448-6698versión impresa ISSN 2007-1124

Resumen

MONTOYA GARCIA, Nancy; PENUELA RIVAS, Claudia Giovanna; ACOSTA DIBARRAT, Jorge Pablo  y  VELAZQUEZ ORDONEZ, Valente. Differential phagocytic activity and in vitro induction of apoptosis by Staphylococcus aureus capsular types 5 and 8 in bovine neutrophils. Rev. mex. de cienc. pecuarias [online]. 2015, vol.6, n.1, pp.99-108. ISSN 2448-6698.

In vitro phagocytosis and apoptosis activity was evaluated in polymorphonuclear leukocytes (PMN) dairy cattle in the presence of Staphylococcus aureus capsular types 5 and 8. PMN were obtained from clinically healthy cows. The phagocytic activity of neutrophils was assayed through the phagocytic capacity and phagocytic index. Apoptosis was determined by light microscopy (LM) and epifluorescence (FM). Differences were observed between treatments in LM regarding the capacity of phagocytosis; compact type (CC) were 86.50±2.93, capsular serotype 5 (CP5) 74.98±2.44 and capsular serotype 8 (CP8) 82.22±1.50 (P<0.05). The values for the phagocytic index were: CC 6.13±0.31, CP5 4.88±0.13 and CP8 5.22±0.10 (P<0.05). The phagocytic capacity in the FM was: CC 86.77±2.6, CP5 62.52±3.26 and CP8 78.60±4.07. The phagocytic index for CC was 7.62±0.55, CP5 4.67±0.29 and CP8 5.53±0.40 (P<0.05). The percentages of apoptosis evaluated by LM were: positive control (C+) 97.7±1.28, negative control (C-) 27.13±1.85, CC 35.44±2.56 CP5 60.07±4.21, CP8 45.57±4.34 and CP5 52.78±3.29. In the FM technique were: C(+) 95.61±2.66, C(-) 20.2±1.77, CC 26.5±1.65, CP8 41.33±2.32 respectively (P<0.05). Differences in the in vitro phagocytosis capacity, phagocytic index and apoptosis of PMN were found between the S. aureus serotypes tested. Type CP5 decreased phagocytic activity and significantly increased the apoptosis compared with type CP8 in PMN.

Palabras llave : Phagocytosis; Apoptosis; Neutrophils; Staphylococcus aureus; Capsular serotypes.

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