Resumen

REZAEI, Mojtaba; MIRHOSSEINI, Seyed Ziaeddin  y  HOSSEIN-ZADEH, Navid Ohavi. PCR-RFLP analysis of leptin gene and its association with milk traits in native cattle of Iran (Bos indicus). Rev. mex. de cienc. pecuarias [online]. 2015, vol.6, n.1, pp.15-24. ISSN 2448-6698.

An experimental cattle population was screened for characterization of gene and genotypic frequencies, and association between BsaAI restriction fragment length polymorphisms in the leptin gene and milk traits. Blood samples were collected from 390 cows provided by census herds of Guilan province in Iran. The extraction of genomic DNA was based on modified Salting Out method. The 522 bp fragment comprising the partial intron 2 and exon 3 region of leptin gene locus was amplified using PCR-RFLP and specific primers. Digestion of amplified fragment with restriction enzyme BsaAI revealed two alleles of A and B with frequencies of 0.447 and 0.553 and three genotypes of AA, AB and BB with frequencies of 0.185, 0.526 and 0.289 in the studied population, respectively. Polymorphism information content was 0.372 and heterozygosity was 0.526. The chi-square test results showed that the studied population was in Hardy-Weinberg equilibrium. The impact of Leptin genotypes on milk traits was conducted based on a linear model of SAS software using least squares means methods. Statistical analyses indicated that the LEP-BsaAI polymorphism has a significant effect on milk yield, milk fat and protein percentage (P<0.01). Animals carrying the heterozygous genotype (AB) produced 1.17 and 0.7 kg/d more milk than the homozygous AA and BB animals, respectively. Animals with the AA genotype had higher fat (0.28 %) and protein (0.17 %) percentage than AB genotype. Such results may be used as a selection criterion to facilitate genetic selection in animal breeding programs.

Palabras llave : DNA polymorphism; Local cattle; Milk yield; RFLP.

        · resumen en Español     · texto en Español     · Español ( pdf )