Abstract

LOPEZ HEYDECK, Sandra Maricruz et al. Porcine reproductive and respiratory syndrome virus (PRRSV) in technified pig farms from the State of México. Rev. mex. de cienc. pecuarias [online]. 2013, vol.4, n.4, pp.469-488. ISSN 2448-6698.

A cluster cross sectional study in a single stage was carried out from April to September 2011 on pigs over 11 wk old from 11 farms, to analyze the viral porcine reproductive and respiratory syndrome (PRRSV) status of technified farms of the State of Mexico. Sera samples (n=220) for ELISA, blood samples (n=80) for one step real-time quantitative reverse transcriptase-PCR (RTqPCR), nasal swabs (n=425) for bacterial isolation of possible porcine respiratory disease complex syndrome (PRDC) pathogens were collected. Additionally information about individuals and farm condition was gathered for further analysis. ELISA shows a 30.67 % PRRSV true seroprevalence been more as likely to be seropositives in farms with PRRSV background and vaccination schedule and of urban-semiurban location; pigs were seroreactive for PRRSV in 7/11 farms (63.63 %). Viremia was found through RTqPCR in 2/80 (2.5 %) of the total of pigs sampled for it, and was in 2/11 farms (18.18 %). We found a sow infected with a European PRRSV that did not show any evident clinical signs of infection, and a sow with a North American PRRSV who displayed clinical signs of the disease. Two farms didn't show seropositive or viremic pigs; these two farms had no PRRSV background or PRRSV vaccination schedule and are located in a semirural area. Staphyloccus aureus and Streptococcus suis where isolated from all the farms and Actinobacillus spp (4/11) or Pasteurella spp (3/11) where found less frequently in some farms. This is the first report in México of a European type PRRSV infection.

Keywords : PRRSV; PCR; ELISA; Bacterial isolation; Porcine respiratory disease complex.

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