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Revista mexicana de ciencias pecuarias

On-line version ISSN 2448-6698Print version ISSN 2007-1124


RUBIO LOZANO, Maria Salud et al. Detection of Listeria monocytogenes, Salmonella and Yersinia enterocolitica in beef at points of sale in Mexico. Rev. mex. de cienc. pecuarias [online]. 2013, vol.4, n.1, pp.107-115. ISSN 2448-6698.

The objective of this project was to detect the presence of Listeria monocytogenes, Salmonella and Yersinia enterocolitica in Mexican and imported beef steaks purchased at supermarkets in three cities of Mexico. Prior to testing beef products, detection limits using PCR-based methods were conducted from both pure bacterial cultures as well as inoculated beef. A total of 90 samples, half of Mexican and half of imported origin, were collected in this study. Overall, a total of 27.78 % of samples tested via PCR were positive for L. monocytogenes, 8.89 % for Salmonella and 28.89 % for Y. enterocolitica, respectively. None of the imported samples tested positive for Salmonella. Mexican samples had a higher Listeria, Yersinia, and Salmonella presence than imported samples, indicating a possible health risk to consumers purchasing this type of product. These results emphasize the need to implement the mandatory use of preventive and control programs, Sanitation Standard Operating Procedures (SSOPs) and HACCP in the Mexican meat industry.

Keywords : Listeria; Salmonella; Yersinia; Beef; PCR; Mexico.

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