Abstract

VALENCIA JUAREZ, María Concepción; ESCOBEDO LOPEZ, Diana; DIAZ ESPINO, Luis Febronio  and  GONZALEZ PEREZ, Enrique. Ex vitro acclimation of Fragaria x ananassa Duch seedlings. Rev. Mex. Cienc. Agríc [online]. 2019, vol.10, n.1, pp.91-100. ISSN 2007-0934.  https://doi.org/10.29312/remexca.v10i1.1633.

In Guanajuato, Mexico, producers propagate strawberry asexually through the use of stolons. This type of propagation is problematic because it facilitates the transmission of diseases directly and indirectly to their descendants. For this reason, the objective of this work was to determine the most favorable environment for the acclimatization of micropropagated strawberry seedlings. For in vitro propagation two culture media with different combinations of growth regulators were evaluated and for acclimation four environments were tested under a completely randomized design. The highest percentage of rooted stems, number of roots and stem length was obtained with LS + 0.2 mg L-1 BA. The best acclimation environment was under 75% shading which promoted the highest survival (91.9%), stem length (28.1), number of basal leaflets (7.7) and total fresh biomass (50.1 g). These results could facilitate the large-scale production of strawberry plants generated under in vitro conditions.

Keywords : environments; hormones; micropropagation.

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