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Revista mexicana de ciencias agrícolas

versão impressa ISSN 2007-0934

Resumo

PEREZ ALVAREZ, Sandra et al. Induced response to antioxidative enzymes in rice under stress due to lead and nickel. Rev. Mex. Cienc. Agríc [online]. 2019, vol.10, n.1, pp.51-62. ISSN 2007-0934.  http://dx.doi.org/10.29312/remexca.v10i1.570.

The effects of lead (PbAc2) and nickel (NiCl2) on the development of rice plants and the activity of antioxidant enzymes, under the same experimental conditions, were evaluated using four concentrations (0, 50, 100 and 300 mg kg-1) in two different organs of the plant (roots and leaves). The research was conducted from September 2010 to March 2011 at the Esalq in Brazil under greenhouse conditions. The activities of the enzymes superoxide dismutase (SOD) and glutathione reductase (GR) were determined by spectrophotocolorimetry and non-denaturing polyacrylamide gel electrophoresis (PAGE) was used to identify the SOD and GR isoenzymes. Rice plants with NiCl2 treatments grew less than those treated with PbAc2 at all concentrations used compared to the control. The highest specific activity of the SOD in the roots was obtained at 0 and 300 mg kg-1 of NiCl2 and 300 mg kg-1 of PbAc2. In leaves, the highest activity of this enzyme was detected with 0 mg kg-1 of NiCl2 and 0 and 300 mg kg-1 of PbAc2. The NiCl2 caused significant increases in GR activity in the roots at all concentrations with respect to the control, no significant differences were observed with respect to the control in any of the organs analyzed in the presence of PbAc2. Four isoenzymes of the SOD (two Mn and two Fe) and five GRs were found in both organs analyzed. Of the latter, the GR-V was identified as leaf tissue specific with the heavy metal Ni.

Palavras-chave : GR; heavy metals; isoenzymes; oxidative stress; rice; SOD.

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