Resumen

RUIZ ANCHONDO, Teresita et al. In vitro establishment of two cultivars released from strawberries: strawberry and raspberry. Rev. Mex. Cienc. Agríc [online]. 2018, vol.9, n.4, pp.799-812. ISSN 2007-0934.  https://doi.org/10.29312/remexca.v9i4.1397.

The development of protocols for the production of healthy plants in vitro is important to satisfy the growing demand for strawberries in northern Mexico as an alternative to traditional fruit trees such as walnut and apple trees. In vitro strawberry and raspberry establishment techniques were evaluated from meristematic explants, apices and internodes. A convective disinfection method was used, where disinfecting agents were used as a 70% ethanolic solution, then they were immersed in a 3% commercial solution solution of sodium hypochlorite: water (1:6), for 15 min. Finally, in 0.05% timerosal® solution for 15 min. Apices were evaluated in the case of strawberry apices, and meristems and internodes in the raspberry. For the establishment, Murashige and Skoog salts added with cytokinin 6-benzylaminopurine (BAP), gibberellic acid (GA) and indole butyric acid (AIB) in different concentrations were used for the strawberry case. In raspberry, MS salts added with BAP, thidiazuron (TDZ), GA and AIB were used. The percentages of establishment, necrosis and contamination were evaluated in both cases. The planting of apices in the case of strawberry and its disinfection system, allowed its establishment in vitro, the variables were analyzed by nonparametric tests showed that the best treatment was TT1 with 70% effectiveness. For raspberries, the case of internodes was better during establishment than in the case of meristems, and the best treatment was TT4, with BAP + GA without auxin, with 57% effectiveness.

Palabras llave : berries; 6-benzylaminopurine; meristems; micropropagation; thidiazuron.

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