Resumo

PEREZ BARRAZA, María Hilda et al. Environment and growth regulators in the expression of Flowering Locus T in mango. Rev. Mex. Cienc. Agríc [online]. 2017, vol.8, n.spe19, pp.3839-3853. ISSN 2007-0934.  https://doi.org/10.29312/remexca.v0i19.653.

The quantification and expression of genes related to flowering is an important tool to understand this process. Studies carried out in Arabidopsis ssp. have served as a basis for molecular studies in fruit trees. The objective was to quantify the expression of the gene flowering locus T (MiFT) in ‘Ataulfo’ mango and its relationship with environment and growth regulators. Two experiments were carried out: 1) trees with 2 500 mg L-1 of paclobutrazol (PBZ) were applied in a single application (1X) at 30 days after pruning (ddp). The mature leaves were collected from September to December 2013 and January to February 2014 under sunny and shaded conditions. Experiment 2: growth regulators, PBZ (2 500 mg L-1, 1X), calcium prohexadione (P-Ca) (500 mg L-1, 3X) and gibberellic acid (250 mg L-1, 1X) were applied. The expression MiFT was quantified, the number and type of growth emerged in flowering, temperature and precipitation were evaluated. The experimental design was completely randomized with factorial arrangement in experiment 1 and completely randomized in experiment 2. MiFT was expressed in all sampled months, the expression varied from 0.3 to 31.4%. On the other hand, when evaluating the expression of the gene with respect to the orientation of the outbreak, the results were 0.081% on the shaded side and 12% on the sunny side. Sunny shoots had the highest flowering ptercentage (66%). MiFT was expressed in leaves treated with PBZ and P-Ca, the highest expression was in December, cooler month, with 4.7 and 30.3%, respectively, which favored flowering. Gibberellins inhibited the expression of the gene and therefore flowering.

Palavras-chave : Mangifera indica L.; flowering; gene expression; growth regulators.

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