Abstract

MONTES-SALAZAR, Alejandra María; SEPULVEDA-JIMENEZ, Gabriela; EVANGELISTA-LOZANO, Silvia  and  RODRIGUEZ-MONROY, Mario. Preliminary study for in vitro propagation of Cedrus atlantica through axillary buds. Rev. Mex. Cienc. Agríc [online]. 2016, vol.7, n.8, pp.2071-2078. ISSN 2007-0934.

Cedrus atlantica is an ornamental plant used to decorate parks and gardens. In order to micropropagate quality material of this plant, an in vitro multiplication protocol was implemented. For this, the axillary buds were used as explants which were disinfected with sodium hypochlorite 10% for 15 min, treatment with which obtained 83% aseptic material. Explants culture was performed in the basal medium McCown’s Woody Plant (WPM) added with activated carbon (1g L-1) and cysteine (25 mg L-1), which allowed to obtain crops without oxidation features. In the multiplication stage with the use of benzyl amino purine (5 mg L-1) obtained up to 5 shoots per explant, which showed an increase of 1.4 cm. However, indole butyric acid at 2.5 and 5 mg L-1 did not induce root formation in vitro shoots.

Keywords : cedar; bud; micropropagation; woody plants.

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