Resumo

PELAEZ-ALVAREZ, Abigail et al. Synergistic effect of Trichoderma asperelleum T8A and captan 50 ^® against Colletotrichum gloeosporioides (Penz.). Rev. Mex. Cienc. Agríc [online]. 2016, vol.7, n.6, pp.1401-1412. ISSN 2007-0934.

The objective of this study was to quantify the interaction Captan 50® - Trichoderma asperelleum T8a as synergistic alternative to control Colletotrichum gloeosporioides ATCC MYA 456, causal agent of anthracnose in mango. The macroscopic, microscopic and molecular characterization confirmed the taxonomy of these organisms as T. asperelleum (T8a) and C. gloeosporioides (ATCC MYA 456), the latter showed the ability to cause anthracnose to 100% of fruits inoculated. The bio-control agent T. asperellum T8a, showed an in vitro inhibition of 91% in the growth of phyto pathogen through parasitic fungi mechanisms. Thus, trials of minimum inhibitory concentration of Captan 50®vs. microorganisms under study were performed, noting that concentrations equal to or greater than 0.5 g L-1 fungicide completely inhibited pathogen growth, however, lower concentrations (0.1 and 0.25 g L-1) inhibited the growth of this only 36 %. Furthermore, T. asperellum T8a growth was inhibited 6% at doses of 0.5 g L-1 of Captan 50®, not observing effects on this strain at lower doses of the fungicide. Thus, the concentration of 0.1 g L-1Captan50® was co-applied in vitro with T. asperellum T8a, observing a synergistic inhibition of C. gloeosporioides ATCC MYA 456, it reached 99%. The data indicate the potential of this integrated alternative to reduce the application of Captan 50®, enhance control of C. gloeosporioides, and reduce economic/environmental problems by the use of this fungicide in field.

Palavras-chave : biological control agent; chemical control; anthracnose; minimum inhibitory concentration.

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