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Revista mexicana de biodiversidad
versão On-line ISSN 2007-8706versão impressa ISSN 1870-3453
Resumo
ALCANTARA-VARGAS, Erik; ESPITIA-LOPEZ, Josefa; GARZA-LOPEZ, Paul Misael e ANGEL-CUAPIO, Alejandro. Conidia production and quality of entomopathogenic strains of the genus Metarhizium anisopliae, isolated in agricultural zones of the State of México. Rev. Mex. Biodiv. [online]. 2020, vol.91, e912912. Epub 22-Dez-2020. ISSN 2007-8706. https://doi.org/10.22201/ib.20078706e.2020.91.2912.
In nature, entomopathogenic fungi are capable of eliminating or reducing agricultural pests and also, they are the most important group for biological control; the main genera used in the industry are: Metarhizium, Beauveria, Isaria and Lecanicillium. The aims of this study were to isolate native strains belonging to Metarhizium anisopliae from agricultural soil in eastern State of México, as well as to evaluate the production, quality and infectivity of conidia obtained in comparison to a commercial product. A completely randomized experimental design was carried out with 3 replicates per sample, where conidia production, viability, germination and infectivity, were measured. The maximum production of conidia was 9 × 108 conidia g of initial dry rice (Oryza sativa) (conidia gidr-1). This represented 1.4 times the order of magnitude than with the commercial product. In all isolates, germination was > 80% and conidia viability was ≈ 50%. Regarding the infectivity parameters, no significant difference was found (p > 0.05) with respect to the commercial product META-SIN®. This method can be an alternative to obtain conidia producing isolates with desirable infectivity.
Palavras-chave : Metarhizium anisopliae; Conidia production; Solid state culture; Quality of conidia; Isolation; Tenebrio molitor.