Resumo

GARCIA-MARQUEZ, Luis Jorge et al. Morphological and molecular identification of Gnathostoma binucleatum (Nematoda: Gnathostomatidae) advanced third stage larvae (AdvL₃) in the state of Colima, Mexico. Rev. Mex. Biodiv. [online]. 2009, vol.80, n.3, pp.867-870. ISSN 2007-8706.

As a part of an ongoing project to understand the current distribution of Gnathostoma species in Mexico, 22 species of vertebrates were examined for this nematode in the state of Colima. The fish species Dormitator latifrons ("chococo") and Sciades guatemalensis ("cuatete") from Cuyutlán Lagoon and the reptile Crocodylus acutus from the Amela Lagoon were positive for infection. Morphometric characteristics of the larvae collected in Colima were similar to those of G. binucleatum larvae collected from other regions and host species in Mexico. The low divergence of the ITS sequence obtained in this study from that of G. binucleatum (0.19%) indicates that our material belongs to this species. This is the first record of the AdvL₃ of G. binucleatum in Colima, the tenth state in Mexico in which this species has been recorded in wild vertebrates.

Palavras-chave : gnathostomiasis; morphometrics; DNA sequences; ITS2; rDNA.

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