SciELO - Scientific Electronic Library Online

 
vol.62 issue2Evaluation of the Role of Matrix Matching for LA-ICP-MS Calibration Approaches in Quantitative Elemental Analysis of Tooth EnamelEvaluation of Polydimethylsiloxane-Phenylsiloxane as a Coating for Stir Bar Sorptive Extraction author indexsubject indexsearch form
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • Have no similar articlesSimilars in SciELO

Share


Journal of the Mexican Chemical Society

Print version ISSN 1870-249X

Abstract

ROCHA, Márcia S. da et al. Single point calibration for quantitative speciation of selenomethionine in yeast Saccharomyces cerevisiae by HPLC-ICP-MS: using reliable, traceable and comparable measurements. J. Mex. Chem. Soc [online]. 2018, vol.62, n.2, pp.334-347. ISSN 1870-249X.  http://dx.doi.org/10.29356/jmcs.v62i2.471.

The production of selenium and selenomethionine certified reference material in yeast (Saccharomyces cerevisiae) and certification studies for its homogeneity, stability and characterization, have been accomplished before in our laboratory. Based on such results and experience of our laboratory in Metrology and according to literature, we discuss here the advantages and limitations of the calibration by “single point” as an alternative method to the classical calibration curve. The method traceability to the SI is checked in order to secure a robust determination. As the model analyte the mass fraction of the Se species selenomethionine is measured and compared in yeast samples. Results showed that a good agreement was obtained by both methods (the reference one of the calibration curve and “single point calibration”). This simple approach can be used for routine control of selenomethionine in commercial supplements of yeast samples, but the work carried out demonstrated its potential for the general routine quantitative analysis of the possible and important selenium species in other Se-enriched supplements commercialised in Latin America (i.e. mushrooms, garlic etc).

Keywords : selenomethionine; calibration curve; single point calibration; measurement uncertainty; HPLC-ICP-MS.

        · abstract in Spanish     · text in English     · English ( pdf )