Abstract

XUE-GUI, Liu et al. Optimization of Extraction Conditions for Flavonoids of Physalis alkekengi var. franchetii Stems by Response Surface Methodology and Inhibition of Acetylcholinesterase Activity. J. Mex. Chem. Soc [online]. 2015, vol.59, n.1, pp.59-66. ISSN 1870-249X.

The microwave-assisted extraction conditions of flavonoids in Physalis alkekengi. var. franchetii stems were optimized using a L3³ Box-Behnken Design. The optimized extraction conditions were determined as follows: 60% for the ethanol concentration, 12.4 for the liquid-to-solid ratio, and 531.4 W for the microwave power, respectively. The yield of the extract obtained under the optimized conditions was 3.85 mg g^-1 which was close to the predicted value. In addition, the extract exhibited potent acetylcholinesterase inhibitory activity, with the IC₅₀ value of 11.61 μg mL^-1 and a maximal inhibition ratio 89.81%. Both the yield and activity were better than reflux extraction.

Keywords : Physalis alkekengi. var. franchetii Stems; Flavonoids; Microwave-assisted Extraction; Response Surface Methodology; Acetylcholinesterase inhibitors.

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