SciELO - Scientific Electronic Library Online

vol.56 issue2Structural and Compositional Optimization of the LiNi0.8Co0.2O2 Electrode by New Synthesis ConditionsReaction of o-Aminobenzamides with Dialkyl Carbonates and Ionic Liquids: A Novel one-pot, High-yield, Microwave-assisted Synthesis of 1-Alkylquinazoline-2,4-diones author indexsubject indexsearch form
Home Pagealphabetic serial listing  

Services on Demand




Related links

  • Have no similar articlesSimilars in SciELO


Journal of the Mexican Chemical Society

Print version ISSN 1870-249X


CANIZARES-MACIAS, María del Pilar; GONZALEZ-SAN MIGUEL, Hilda María  and  CERDA, Víctor. Implementing Stepwise Solvent Elution in Multisyringe Liquid Chromatography (MSC) to Separate Norepinephrine, Epinephrine and Dopamine. J. Mex. Chem. Soc [online]. 2012, vol.56, n.2, pp.194-200. ISSN 1870-249X.

A multisyringe liquid chromatographic (MSC) method by using stepwise solvent elution and reverse-phase liquid chromatography C18 monolithic column to separate Epinephrine (E), Norepinephrine (NE) and Dopamine (DA) was developed. The first step employs the mixture sodium1-hexanosulfonate monohydrate (HS) 7.5mM: methanol (98:2) to separate NE and E and the second step uses HS (5.0 mM): methanol (92:8) to elute DA. The three analytes were separated on 6 min spending only 10 mL of mobile phases. The detection limits were between 1.3 μg mL-1 (DA) and 2.04 μg mL-1 (NE).

Keywords : Multisyringe liquid chromatography; C18 monolithic column; catecholamines; stepwise elution.

        · abstract in Spanish     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License