SciELO - Scientific Electronic Library Online

vol.56 número1Determination of Antioxidants in Fruit Tissues from Three Accessions of Habanero Pepper (Capsicum chinense Jacq.)Metal Binding Sites in Plant Soluble Inorganic Pyrophosphatases: An Example of the Use of ROSETTA Design and Hidden Markov Models to Guide the Homology Modeling of Proteins índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados

  • No hay artículos similaresSimilares en SciELO


Journal of the Mexican Chemical Society

versión impresa ISSN 1870-249X


GUIZAR-GONZALEZ, Cecilia; TRUJILLO-VILLANUEVA, Karen; MONFORTE-GONZALEZ, Miriam  y  VAZQUEZ-FLOTA, Felipe. Sanguinarine and Dihydrosanguinarine Accumulation in Argemone mexicana (L) Cell Suspension Cultures Exposed to Yeast Extract. J. Mex. Chem. Soc [online]. 2012, vol.56, n.1, pp.19-22. ISSN 1870-249X.

Alkaloid contents in Argemone mexicana cell line AMMiF were analyzed during a 36-day culture cycle. The benzophenanthridine sanguinarine (SA) represented the main alkaloid. Dihydrosanguinarine (DHSA), a SA biosynthetic precursor and a less toxic benzophenanthridine, was also identified, based on chromatographic properties and further confirmed by gas chromatography coupled to mass spectrometry. Along the culture cycle, SA contents increased simultaneously while DHSA decrease, suggesting their biosynthetic conversion. Upon exposure to yeast extract as an inducer of secondary metabolism, there was an increase in SA content, which was preceded by DHS accumulation. However, after a long exposure (72 h), contents of both SA and DHSA were noticeable elevated, with close to 20% of the total alkaloid produced being recovered from the external medium. These results suggest the operation of a cell mechanism to avoid damage inflicted by the excessive accumulation of SA.

Palabras llave : Argemone mexicana; benzophenathridines; dihydrosanguinarine; sanguinarine.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons