Abstract

GUERRERO-GERMAN, Patricia; LUCERO-ACUNA, Armando; MONTESINOS, Rosa Ma.  and  TEJEDA-MANSIR, Armando. Analysis and Simulation of Batch Affínity Processes Applied to Separation of Biomolecules. J. Mex. Chem. Soc [online]. 2007, vol.51, n.2, pp.59-66. ISSN 1870-249X.

The scale-up and optimization of large-scale affinity chromatographic operations is of major industrial importance. In this work, a transport model which includes pore diffusion, external film resistance, and finite kinetic rate, was used to mathematically describe the performance of a batch affinity adsorption system. Experimental data from literature describing the adsorption of β-galactosidase onto anti-β-galactosidase immobilized on porous silica was used as a model system. The mathematical model was solved using the numerical method of lines (MOL) in a MATLAB platform. The use of the transport model is a unique way to predict batch affinity performance as well as to obtain a better understanding of the fundamental mechanisms involved in the bioseparations.

Keywords : Mathematical modeling; batch affinity chromatography; biomolecules.

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