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Journal of the Mexican Chemical Society

Print version ISSN 1870-249X


VERA-AVILA, Luz E.; MACIAS-MENDOZA, Diana; COVARRUBIAS-HERRERA, Rosario  and  VELAZQUEZ-RAMIREZ, José G.. On-line Solid-Phase Extraction-Liquid Chromatographic Method for the Determination of Carbofuran and 7-Phenolcarbofuran in Cervical-Uterine Epithelial Tissue. J. Mex. Chem. Soc [online]. 2005, vol.49, n.1, pp.32-38. ISSN 1870-249X.

A method for the trace level determination of carbofuran and 7-phenolcarbofuran in cervical-uterine epithelial tissue samples is proposed. The finely chopped tissue is methanol extracted in an ultrasonic bath; the extract is evaporated, diluted with water and acidified to eliminate hydrophobic impurities by precipitation. Preconcentration and additional cleanup are carried out in a polymeric (PLRP-S) precolumn by loading the filtrated extract and successively washing with acetonitrile-water 5:95 (v/v) and 0.01 M aqueous ammonia. The precolumn is finally on-line eluted and analysed by reverse-phase HPLC with UV detection at 280 nm. Recoveries >90% and relative standard deviation of ∼12% were obtained in the analysis of non-contaminated tissue samples spiked at 0.2 µg/g of the pesticide and metabolite. Absolute limits of detection were 20 ng of analyte in a cervical biopsy (150-950 mg of epithelial tissue). Application to the analysis of three samples from countrywomen exposed to carbofuran revealed the probable presence of 7-phenolcar-bofuran in two of them.

Keywords : Carbofuran; 7-phenolcarbofuran; trace analysis; cervical-uterine tissue.

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