Services on Demand
Journal
Article
Indicators
- Cited by SciELO
- Access statistics
Related links
- Similars in SciELO
Share
Revista odontológica mexicana
Print version ISSN 1870-199X
Abstract
MADERA ANAYA, Meisser Vidal and SUAREZ CAUSADO, Amileth. Evaluation of two RNA extraction methods in children's saliva. Rev. Odont. Mex [online]. 2017, vol.21, n.4, pp.245-252. ISSN 1870-199X.
Objective:
To standardize an RNA (ribonucleic acid) extraction protocol in children's saliva specimens.
Methods:
The study was conducted on saliva specimens from 60 children who participated in the study with their parents' authorization. Comparison of two RNA extraction methods was established; methods assessed concentration, quality and yield. Moreover, genie expression of GUSB gene was achieved with RT-PCR (reverse transcriptase-polymerase chain reaction). Data were analyzed through measurements of central tendency and dispersion, frequencies and percentages. Т-Student and χ2 tests were used in order to differentiate between both methods (p < 0.05).
Results:
Analysis of cell amounts per saliva ml revealed a mean of 564,977.8 (SD = 246,678.6); a RNA in children's saliva extraction method was standardized, specifically the method using RNeasy® Protect Saliva Mini Kit Qiagen exhibited better characteristics of RNA concentration (p = 0.0000) and yield (p = 0.0000) when compared to the method using QIAzór®; no RNA (p = 0.146) quality differences were found.
Conclusions:
RNA could be extracted from children's saliva specimens, therefore, it is suggested to use saliva for the molecular analysis of different oral and systemic diseases.
Keywords : Saliva; RNA; transcriptome; biomarkers.