Tropical and subtropical agroecosystems
versión On-line ISSN 1870-0462
PADILLA-CAMPOS, Isaac F. et al. Molecular pattern of Mycobacterium bovis isolates and its relationship with risk factors associated with the presence of bovine tuberculosis in northern Mexico. Trop. subtrop. agroecosyt [online]. 2011, vol.14, n.2, pp.465-471. ISSN 1870-0462.
The objective of this study was to determine the molecular pattern of M. bovis isolates from cattle of Northern Mexico and its relationship with some risk factors. Isolates (n=60) were obtained from the states of Coahuila (COA, n=14), Tamaulipas (TAM, n=16), Nuevo Leon (NL, n=14) and, Baja California and Durango (DUR, n=16). The risk factors studied were: system of production (Dairy and beef), state, age, lesion type (localized and generalized), and type of presentation (caseous and calcified). Samples were analyzed at the Regional Laboratory of Monterrey NL, following a spoligotyping protocol. Twenty-five spoligotypes belonging to the M. bovis complex were identified. Eleven (18.3%) isolates presented a unique pattern, whereas 49 (81.7%) were grouped in 14 clusters. The largest clusters had 12 and 17 isolates. The average heterocigosities per state were 21.4% (NL), 15.6% (TAM), (15.6% COA) and 9.9% (DUR). The genetic distances of the isolates between states did not show differences (P > 0.05) when examined by Chi-square tests. The average genetic diversity (15.6%) was due to the variation of strains within subpopulations. In this study an 8.3% difference among states was obtained, which suggest the idea of a unique strain of M. bovis with many variants and that the genetic diversity found for M. bovis could be in part to animal movement between regions. Statistical analysis did not show association (P > 0.05) between risk factors and strains ofM. bovis.
Palabras llave : Bovine tuberculosis; Spoligotypes; M. bovis; Risk factors.