SciELO - Scientific Electronic Library Online

 
vol.14 número3Efecto de la suplementación mineral y el tipo de almidón en la producción de prodigiosina a partir de un cultivo de Serratia marcescens BS303Evaluación de la estabilidad de nanoemulsiones de β -Caroteno preparadas por un método de homogeneización -Emulsificación empleando ácido esteárico como fase oleosa índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay artículos similaresSimilares en SciELO

Compartir


Revista mexicana de ingeniería química

versión impresa ISSN 1665-2738

Resumen

VELASCO-BUCHELI, R. et al. Transformation of trans-Anethole using the plant pathogenic fungus Colletotrichum acutatum as biocatalyst. Rev. Mex. Ing. Quím [online]. 2015, vol.14, n.3, pp.653-666. ISSN 1665-2738.

Microbial transformation of propenylbenzenes may offer a cleaner and cheaper alternative to natural production of flavors and fragrances. In the present study, the biotransformation of trans-anethole using cells of a Colombian strain of the fungus Colletotrichum acutatum was investigated. Initially, fungitoxicity of this compound against C. acutatum was evaluated; trans-anethole displayed a relatively weak toxiciiy against the microorganism (<70%, at 200 μg/mL and after 48 hours) and apparently a detoxification mechanism was present. Then, the microorganism was incubated with the substrate atroom conditions, using three different culture media (Czapek-Dox, Sabouraud and PDB). Results show that trans-anethole is mainly degraded through an epoxide-diol pathway (trans-anethole to anethole-epoxide, then to syn- and anti-anethole-diol, p-anisaldehyde, p-anisic acid and p-anisic alcohol). However, other minor metabolites [e.g. 3-(4-methoxyphenyl)-1-propanol, 1-(4-methoxyphenyl)-2-propanol, ethyl ester of anisic acid], possibly proceeding from other metabolic pathways were also found. Additionally, it was demonsfrated that: the concenfration of metabolic products is dependent on culture medium used, being anethole-diol the mapr product obtained in all media used. Interestingly, some of the compounds generated in the biotransformation have been utilized as flavors and fragrances. Based on the identified metabolites, a possible metabolic pathway of the biotransformation of trans-anethole by C. acutatum was proposed.

Palabras llave : fungitoxicity; biotransformation; C. acutatum; metabolic pathway; culture media.

        · resumen en Español     · texto en Inglés     · Inglés ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons