Resumen

REYES-MENDEZ, A.I. et al. Production of calcium- and iron-binding peptides by probiotic strains of Bacillus subtilis, B. clausii and B. coagulans GBI-30. Rev. Mex. Ing. Quím [online]. 2015, vol.14, n.1, pp.1-9. ISSN 1665-2738.

Some strains of Bacillus subtilis, B. clausii and B. coagulans are commercially used as probiotic bacteria and their proteolytic activity is well known. The aim of this work was to evaluate their capacity to produce calcium- and iron-binding peptides when grown in culture media with different nitrogen sources. The fermentation media included broths containing tryptic soy (TS), casein peptone (CP), soy peptone (SP), and a crude phycobiliprotein extract (CPE). Cell-free supernatants (CFS) were prepared from the fermented media after 24, 48 and 72 h of fermentation and, tested for degree of hydrolysis (DH). Calcium- and iron-binding activities were determined as well. When the inocula were prepared in a medium designed for the production of alkaline proteases (AP), the degree of hydrolysis and the mineral-binding activity in the CFS were higher. The best rerults for calcium-binding activity and DH were obtained when B. subtilis and B. coagulans grew in a CPE-containing medium. Analogous results were found for trie iron-binding activity; nevertheless, this bioactivity was also high for B. clausii when grown in broths containing TS-, CP- and, SP.

Palabras llave : bioactive peptides; Bacillus subtilis; Bacillus clausii; Bacillus coagulans; calcium-binding; iron-binding; proteolysis; spirulina.

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