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Revista mexicana de ingeniería química

versão impressa ISSN 1665-2738

Resumo

LARA, Álvaro R.. Recombinant protein production in Escherichia coli. Rev. Mex. Ing. Quím [online]. 2011, vol.10, n.2, pp. 209-223. ISSN 1665-2738.

Recombinant proteins production (R P) is one of the most important contributions of modern biotechnology. The most important bacterial host for RP production is Escherichia coli, although B acillus subtilis and Bacillus megaterium are also gaining importance. Due to its economical relevance and impact on human health, the present review is foc used on the production of RP in E. coli. General aspects of vector design, strains election as well as cultivation and downstream processing are covered. Particularly, the opportunities to improve cultivation schemes and downstream through metabolic engineering are discussed.

Palavras-chave : recombinant proteins; Escherichia coli; metabolic engineering; bioreactors.

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