Abstract

OROZCO-SANCHEZ, F  and  RODRIGUEZ-MONROY, M. Cell suspension culture of Azadirachta indica for production of a bioinsecticide. Rev. Mex. Ing. Quím [online]. 2007, vol.6, n.3, pp.251-258. ISSN 1665-2738.

Seeds of Azadirachta indica are an important source for production of bioinsecticides, being Azadirachtin (Aza) the main compound. However, agricultural and climate adversity limit the seed production, thereby plant cell culture in vitro is an alternative technology for Aza production. In this work, the state of the knowledge of A. indica cell culture is presented and the strategies for improving growth and production of Aza are analyzed. The use of cell membrane permeabilizing agents to liberate intracellular Aza, of biosynthetic precursors, light, temperature, and the effect of media culture are revised. The stirred tank bioreactor has been the most used for cell culture and the studies have been centered on impellers evaluation and the possibility of using the fed-batch regime. Calculated productivities show that the bioreactor is technologically appropriated for cultivation of A. indica cell cultures and bioinsecticide production.

Keywords : Azadirachta indica; Azadirachtin; bioreactor; bioinsecticide.

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