Resumo

CAMACHO-JIMENEZ, Laura et al. Dose-dependent effect of recombinant CHH-B1 on osmoregulatory capacity and Na⁺/K⁺-ATPase expression in bilaterally eyestalk-ablated shrimp Litopenaeus vannamei. Biotecnia [online]. 2021, vol.23, n.3, pp.58-65.  Epub 07-Mar-2022. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v23i3.1441.

Osmoregulation in crustaceans is under neuroendocrine control by the crustacean hyperglycemic hormones (CHHs), which modify water and ion concentrations in diverse species. Previous studies suggested that CHH variant B1 (CHH-B1) has effects on the osmoregulatory responses of the Pacific white shrimp Litopenaeus vannamei. For a better understanding of the molecular action mechanisms of CHH-B1 in osmoregulation, in this work, a biological assay was done by injecting different dosages (5, 10, 50, 100, 250, 500, and 1000 pmol) of recombinant CHH-B1 (rCHH-B1) into bilaterally eyestalk-ablated shrimp under hyper-osmotic conditions. The gene expression of Na+/K+-ATPase (NKA) was quantified by RT-qPCR in gills of shrimp injected with rCHH-B1. In addition, osmoregulatory capacity (OC) of shrimp was estimated as the difference between the osmotic pressure (OP) of the external medium and that of hemolymph. The results indicate that CHH-B1 regulates the OC of shrimp during hypo-regulation by modulating Na+/K+-ATPase at transcriptional level in a dose-dependent way. Our results suggest that CHH has direct participation in the control of osmo-ionic regulation mechanisms, not only in L. vannamei but in crustaceans in general.

Palavras-chave : Litopenaeus vannamei; shrimp; crustacean hyperglycemic hormone; osmoregulatory capacity; Na⁺/K⁺-ATPase.

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