Abstract

SANCHEZ MENDOZA, Ana Elvia et al. Evaluation of the effect of G530A (calpain), C357G (calpastatin), G1795A (myopaladin) and G1181A (PPARGC1A) SNPs, on beef tenderness of a region of Veracruz State, Mexico. Biotecnia [online]. 2021, vol.23, n.1, pp.13-20.  Epub June 18, 2021. ISSN 1665-1456.  https://doi.org/10.18633/biotecnia.v23i1.1098.

Tenderness, an important characteristic for marketing beef, has been associated with genetic factors, such as single nucleotide polymorphisms (SNPs). The aim of this work was to analyze the genotypic and allelic distribution of G530A, C357G, G1795A and G1181A SNPs to establish an association with meat tenderness. Ninety samples of longissimus dorsi muscle were obtained. Tenderness was determined as a Warner-Bratzler shear force. SNPs genotyping was performed by PCR-RFLP. Using a generalized linear model (GLM), we evaluated the association between SNPs and tenderness. The effect of the alleles was determined by allelic substitution analysis. It was determined that the genetic group influenced tenderness (p <0.05). For C530A and G1181A SNPs, a lower frequency was obtained for the AA genotypes, while for C357G and G1795A SNPs higher frequencies were observed for the ancestral CC and GG genotypes. We found no genotypic or allelic association between SNPs and tenderness. This shows the importance of carrying out regional analysis of association between SNPs and quality characteristics to introduce them favorably through marker-assisted selection programs.

Keywords : SNPs; PCR-RFLP; cattle; meat quality; tenderness.

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