Resumen

PARRA-ORTEGA, Israel; VILCHIS-ORDONEZ, Armando; LOPEZ-MARTINEZ, Briceida  y  ANGELES-FLORIANO, Tania. Analytical recommendations for SARS-CoV-2 identification by RT-PCR in pediatric patients. Bol. Med. Hosp. Infant. Mex. [online]. 2021, vol.78, n.3, pp.171-180.  Epub 25-Jun-2021. ISSN 1665-1146.  https://doi.org/10.24875/bmhim.20000210.

Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome 2 coronavirus (SARS-CoV-2) and is currently listed as a global public health emergency. Timely identification and protocol implementations for molecular detection of this virus are vital for medical decision-making. Identification of SARS-CoV-2 infection cases is based on detection of the virus RNA by molecular tests, particularly real-time reverse transcription-polymerase chain reaction (RT-PCR). Technical and operational details specific to each center must be considered to perform the molecular diagnosis of SARS-CoV-2 in pediatric patients. The term “qualified laboratories” involves laboratories in which all users, analysts, and anyone reporting results are trained to develop and interpret results through a procedure implemented previously by an instructor. Such knowledge is essential in detecting and identifying errors during each of its phases: pre-analytical, analytical, and post-analytical, which allow the establishment of continuous improvement policies to ensure the quality of the results, but above all, the physical integrity of health workers.

Palabras llave : COVID-19; SARS-CoV-2; RT-PCR.

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