SciELO - Scientific Electronic Library Online

 
vol.76 número2La crítica y la educación médica: hacia un conocimiento liberador. Parte IIFactores asociados con la picadura de alacrán en escolares: estudio transversal en dos comunidades rurales de Guerrero, México índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay artículos similaresSimilares en SciELO

Compartir


Boletín médico del Hospital Infantil de México

versión impresa ISSN 1665-1146

Resumen

PARRA-ORTEGA, Israel et al. Detection and quantification of T-cell subpopulations and NK cells in peripheral blood from healthy individuals. Bol. Med. Hosp. Infant. Mex. [online]. 2019, vol.76, n.2, pp.66-78. ISSN 1665-1146.  http://dx.doi.org/10.24875/bmhim.18000083.

Background:

The knowledge of the participation of different subpopulations of T lymphocytes in various pathologies helps to make therapeutic decisions, having as reference the presence of the different subpopulations of the T lymphocytes associated with the disease.

Methods:

A profile standardization of surface molecules for the characterization of subpopulations of T cells was conducted: naïve, activated and memory, as well as natural killer (CD3− CD56+) cells in peripheral blood of clinically healthy individuals.

Results:

Naïve (CD3+, CD4+ or CD8+, CD45RA+, CD62L+, CCR7+), activated (CD3+, CD4+ or CD8+, CD45RA+ or CD45RO+, CD69+ and/or CRTAM+), effectors (CD3+, CD4+ o CD8+, CD45RA+, CD62L−, CCR7−), central memory (CD3+, CD4+ o CD8+, CD45RO+, CD62L+, CCR7+), memory effectors (CD3+, CD4+ or CD8+, CD62RO+, CD62L−, CCR7−) subpopulations were analyzed by flow cytometry. Descriptive statistics parameters were calculated (minimum values, maximum values, mean values, median).

Conclusions:

This panel can be very useful for monitoring patients in whom the immunological status from a cellular perspective is needed. Particularly, it can support the follow-up of patients who require an immunological reconstitution (T-cell component) evaluation.

Palabras llave : T lymphocyte; Lymphocyte subpopulation; Flow cytometry.

        · resumen en Español     · texto en Español     · Español ( pdf )