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Boletín médico del Hospital Infantil de México

versión impresa ISSN 1665-1146

Resumen

HERNANDEZ-LUNA, Marco Antonio et al. Bactofection of sequences encoding a Bax protein peptide chemosensitizes prostate cancer tumor cells. Bol. Med. Hosp. Infant. Mex. [online]. 2016, vol.73, n.6, pp.388-396. ISSN 1665-1146.  https://doi.org/10.1016/j.bmhimx.2016.10.002.

Background:

Tumor cell resistance to chemotherapy agents is one of the main problems in the eradication of different neoplasias. One of the mechanisms of this process is the overexpression of anti-apoptotic proteins such as Bcl-2 and Bcl-XL; blocking the activity of these proteins may contribute to the sensitization of tumor cells and allow the adequate effects of chemotherapeutic drugs.

Methods and results:

This study adressed the transfection of prostate cancer cells (PC3) with a plasmid encoding a recombinant protein with an antagonist peptide from the BH3 region of the Bax protein fused to the GFP reporter protein (BaxGFP).

This protein induced apoptosis of these tumor cells; further, selective transport of this plasmid to the tumor cell with Salmonella enterica serovar Typhimurium (strain SL3261), a live-attenuated bacterial vector, can induce sensitization of the tumor cell to the action of drugs such as cisplatin, through a process known as bactofection.

Conclusions:

These results suggest that Salmonella enterica can be used as a carrier vector of nucleotide sequences encoding heterologous molecules used in antitumor therapy.

Palabras llave : Bactofection; Bax peptides; Salmonella; Cancer therapy.

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