SciELO - Scientific Electronic Library Online

 
vol.67 número5Enfoque integral del consumo y administración de drogas en edad pediátrica en la población mexicanaEfectividad del ácido ursodesoxicólico versus fenobarbital para el tratamiento de la colestasis en prematuros. Ensayo clínico cruzado índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay artículos similaresSimilares en SciELO

Compartir


Boletín médico del Hospital Infantil de México

versión impresa ISSN 1665-1146

Resumen

PARRA-ORTEGA, Israel et al. Atypic expression in the detection and quantification of Epstein-Barr virus using real-time PCR. Bol. Med. Hosp. Infant. Mex. [online]. 2010, vol.67, n.5, pp.416-421. ISSN 1665-1146.

Background. Epstein-Barr virus (EBV) viral load is useful not only for detection of an active infection but also as a tumor marker for certain malignant forms. In immunocompromised patients it is related to postransplant lymphoproliferative syndrome (PTLS) with an incidence on the order of 1 to 20%. It is recommended to determine viral load in whole blood and plasma to monitor patients at risk for developing PTLS. Real-time polymerase chain reaction (RT-PCR) is a useful tool for this determination. In these determinations the melting curve (Tm) plays an important role because changes in Tm suggest that the target sequence has suffered mutations, although the selected regions for detection and quantification of EBV are highly conserved. We undertook this study to describe the findings of the variations identified in the EBV genome and to perform the detection and quantification in samples of pediatric patients using RT-PCR. Methods. Results from 352 pediatric patients were analyzed retrospectively in whom investigation of the EBV was performed in peripheral blood and plasma by RT-PCR. For detection and quantification of EBV, a 166-bp fragment of the genome was amplified using a design of TIB MOLBIOL and the LightCycler® equipment with a Tm of 68 °C. Results. Of the 352 patients studied, in 132 (37.5%) presence of EBV was detected and quantified the viral load. In five (3.8%) of the positive patients, a change of the Tm was identified Using electrophoresis running in agarose gel, it was proved that the obtained amplification corresponds to the 166-bp fragment. Conclusion. The specific product and size of the amplified remained unchanged; therefore, we there is a high probability of decrease in the concentration of guanine-cytosine in the target sequence because the Tm showed a decrease in all the reported cases. It is required the sequence of the amplification is required to precisely determine the cause of the decrease in the Tm.

Palabras llave : viral load; immunocompromised patients; posttransplant lymphoproliferative syndrome; Epstein-Barr virus.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons